Staining of .221 cells expressing various members of the CEACAM family using specific anti-CEACAM antibodies. We generated .221 transfectants as described in Methods. Each row shows the staining performed on a particular transfectant (indicated at left), and each column shows the staining with a particular antibody (indicated at top). Bold lines represent antibody staining and thin lines show background staining on .221 cells. One representative experiment is shown out of three performed.