IC-loaded BMDCs induce OVA-specific cellular and humoral immunity in vivo. (a) OVA–peptide MHC class I– and II–restricted cellular immune responses result from OVA/IC–loaded BMDC immunization. Proliferative responses occur after addition of MHC class I– or class II–restricted peptides to lymph node cell populations from mice immunized with BMDCs loaded with OVA/ICs but not OVA alone. (b) Antibody responses are enhanced after immunization with OVA/IC–loaded BMDCs. OVA-specific ELISA reveals higher OVA-specific total IgG, IgG1, and IgG2a titers in diluted sera from mice immunized 15 days earlier with IC-pulsed rather than OVA-pulsed BMDCs (ten mice per group).