Neutrophil-depleted mice have slight functional protection against ischemic ARF (a) and are not histologically protected against ischemic ARF (b). (a) There was an increase in serum creatinine in vehicle-treated mice with ischemic ARF (neutro+). Neutrophil-depleted mice with ischemic ARF (neutro–) had a slight decrease in serum creatinine compared with neutro+ mice. *P < 0.001 vs. sham; **P < 0.05 vs. neutro+ ARF, P < 0.001 vs. sham; n = 8. (b) Histological scoring of ATN in the outer medulla was the same in neutro+ as in neutro– mice. *P < 0.001 vs. sham; **P < 0.001 vs. sham, NS vs. neutro+; n = 7. The lack of protection occurs despite the prevention of neutrophil infiltration in the kidney in mice with ischemic ARF, shown in Figure 6c.