Mice treated with OPH-001 have decreased caspase-1 activity (a), IL-18 protein (b), and neutrophil infiltration (c) in the kidney in mice with ischemic ARF. (a) OPH-001 resulted in a normalization of caspase-1 activity compared with that of vehicle-treated mice. *P < 0.05 vs. sham; **P < 0.05 vs. vehicle-treated ARF, NS vs. sham; n = 4. (b) IL-18 protein (ECL assay) was increased in ischemic ARF. OPH-001 resulted in a normalization of IL-18 compared with that of vehicle-treated mice. *P < 0.01 vs. sham; **P < 0.01 vs. vehicle-treated ARF, NS vs. sham; n = 4. (c) Neutrophil infiltration (neutrophils/mm²) was increased in vehicle-treated mice with ischemic ARF and prevented in OPH-001–treated mice with ischemic ARF. *P < 0.01 vs. sham; **P < 0.01 vs. vehicle-treated ARF, NS vs. sham; n = 5.