Control and VFEpoR mice were fed a Western diet and treated with LDLR ASO and EPO for 12 weeks, with Liprox-1 (10 mg/kg, 3 times per week) or vehicle injection for the last 10 weeks. (A) Experiment timeline. (B) Representative C11 BODIPY staining histogram and quantification of C11 BODIPY CD11b⁺ cells as the percentage of total aortic CD11b⁺ cells. (C) H&E-stained images of aortic root sections. Necrotic core regions indicated by broken lines and quantification of total lesion area and necrotic core area are shown. Scale bar: 200 μm. (D) Aortic root sections were stained with Masson’s trichrome staining for fibrous cap (red, outlined by broken lines) and collagen (blue) content area, and then quantified as the ratio of total lesion area. Scale bar: 100 μm. (E) 4-HNE IHC in the aortic root cross sections. Scale bar: 100 μm. (F) Representative images of Perl’s blue plus DAB staining, IHC staining of RBCs (anti-Ter119), and macrophages (anti-Mac2) in aortic roots. Bar graph shows quantification of iron (II + III)–positive and erythrophagocytosis (Ter119⁺Mac2⁺) cell counts per section. Scale bar: 100 μm. (G) Immunofluorescence staining of TfR1 and macrophage (anti-Mac2), and quantification of TfR1 and macrophage costaining cell counts per section. Scale bar: 50 μm. (H) Evans blue intravital staining of arches and quantification of Evans blue extravasation. (I) L-selectin and CD11a expression levels in neutrophils and monocytes from peripheral blood assessed by flow cytometry. (J) VFEpoR mice were fed a Western diet and injected with LDLR ASO and EPO for 3 weeks, then divided into two groups, treated with Isotype or anti–Gr-1 mAb twice per week for another 4 weeks. In total 7 weeks WD and EPO injection. Evans blue intravital staining of arches and descending aorta, and quantification of extravasation. One-way ANOVA (B–D) or unpaired 2-tailed t test (E–J). *P < 0.05, **P < 0.01, ***P < 0.001.