E₂ and OHT differentially modulate ER signaling on the hTERT promoter and on telomerase activity in LNCaP cells. (a) Cotransfections of p1009 reporter alone (–ER) or in combination with expression vectors for ER-α (+ER-α) or ER-β (+ER-β) were performed with or without ligand (E₂ or OHT). Results represent the average (± SEM) of four independent experiments, each performed in duplicate, and values are expressed as fold induction (+/– ligand), as described in the legend to Figure 3. (b) ChIP assay was performed in LNCaP untreated (–) or treated (+) with E₂, OHT, or T₃, as described in the legend to Figure 3. (c) Extracts (0.25 μg) from cells treated with OHT for 0, 3, 6, and 24 hours were assayed for telomerase activity by TRAP.