CLA expression by circulating CD8⁺ lymphocytes specific for human herpesviruses. Subject ID numbers (Table 1) are included for HSV-2 analyses. (a) Tetramer B7-RPR-PE and anti-CD8α staining of lymphocytes in PBMCs from HSV-2–infected, B7 subjects. The percentages of CD8α-high lymphocytes staining with the tetramers are given in Figure 1i. Quadrants show criteria for CD8α-high cells and tetramer binding. (b) Expression of CLA by CD8α-high lymphocytes staining with tetramer B7-RPR-PE. The percentage of CLA-positive cells is indicated. (c) Expression of CLA by CD8α-high lymphocytes that do not stain with tetramer B7-RPR. (d) Staining of PBMCs from an HLA A2 subjects with anti-CD8α and tetramers A2-GLA specific for HSV-2 (panel 1), A2-VLE-PE or A2-NVP-PE specific for CMV (panels 2 and 3), or A2-CLG-PE or A2-GLC-PE specific for EBV (panels 4 and 5). For subject 10433, the gates for tetramer-high and tetramer-low CD8⁺ cells are shown. The proportion of CD8α-high cells staining with tetramer and the quadrant criteria for CD8α-high cells and tetramer binding are shown. The CMV and EBV data correspond to lines 5, 1, 10, and 12 of Table 2. (e) Expression of CLA by CD8α-high lymphocytes that stain with herpesvirus tetramers. (f) Expression of CLA by CD8α-high lymphocytes not staining with the indicated tetramers. Percentages of CLA-positive cells are indicated.