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Evidence that fibroblasts derive from epithelium during tissue fibrosis
Masayuki Iwano, … , Hirokazu Okada, Eric G. Neilson
Masayuki Iwano, … , Hirokazu Okada, Eric G. Neilson
Published August 1, 2002
Citation Information: J Clin Invest. 2002;110(3):341-350. https://doi.org/10.1172/JCI15518.
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Categories: Article Hematology

Evidence that fibroblasts derive from epithelium during tissue fibrosis

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Abstract

Research Article

Authors

Masayuki Iwano, David Plieth, Theodore M. Danoff, Chengsen Xue, Hirokazu Okada, Eric G. Neilson

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Figure 1

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Characterization of γGT.Cre mice. (a) Plasmid map of the γGT.Cre transge...
Characterization of γGT.Cre mice. (a) Plasmid map of the γGT.Cre transgene injected into B6 × SJL zygotes for the production of transgenic mice. (b) γGT.Cre transgenic mice express Cre transcripts only in the kidney and after postpartum day 7 (day P7), shown by Northern blot probed with cDNA encoding Cre and GAPDH. B, day of birth. (c) Immunofluorescent staining of a paraffin-embedded kidney section from γGT.Cre transgenic mice using antibody against Cre demonstrates staining in cortical proximal tubules but not in the medulla by confocal microscopy (×400); inset at higher power shows cortical tubular (CT) staining (×630). (d) R26R × γGT.Cre F1 hybrid mice express a recombination amplicon of approximately 575 bp’s (shown by PCR) (7, 8) in the kidney but not in the liver. bpA, poly A tail; Br, brain; Lu, lung; Ad, adrenal gland; Lv, liver; Sp, spleen; Mu, muscle; Kd, kidney.
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