Joost P. Schanstra, Eric Neau, Pascale Drogoz, Miguel A. Arevalo Gomez, José Miguel Lopez Novoa, Denis Calise, Christiane Pecher, Michael Bader, Jean-Pierre Girolami, Jean-Loup Bascands
Expression and activity of the renal plasminogen system in 5 day–obstructed and sham-operated B2+/+ and B2–/– mice. (a) Northern blot mRNA expression analysis. Representative autoradiograms of tPA, uPA, PAI-1, and GAPDH are shown. Adjacent histograms are the mean densitometric values (± SD) after analysis of the Northern blots (n = 10) and normalization with GAPDH. (b) PA activity. (c) Analysis of matrix MMP-2 activity by zymography. Quantification of MMP-2 activity by densitometric analysis is shown in the histogram. *P < 0.05 compared with respective sham-operated mice. ‡P < 0.05 compared with B2+/+ UUO mice. L, latent; A, active MMP-2.