IL-2 induces RANTES and RFLAT-1 expression. (a) IL-2–dependent T cells were stimulated with 1,000 U/ml rIL-2, and intracellular RANTES was measured by FACS. Dotted line, no stimulation; solid line, 4 hours after stimulation. (b and c) Aliquots of the cells were lysed and subjected to Western blot analysis using anti–RFLAT-1, anti-eIF4E, and anti-Hsc70 Ab. Results are representative of two independent experiments. *P < 0.05.