Heparin’s anti-inflammatory effects require glucosamine 6-O-sulfation and are mediated by blockade of L- and P-selectins
Lianchun Wang, … , Ajit Varki, Jeffrey D. Esko
Lianchun Wang, … , Ajit Varki, Jeffrey D. Esko
Published July 1, 2002
Citation Information: J Clin Invest. 2002;110(1):127-136. https://doi.org/10.1172/JCI14996.
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Article Vascular biology

Heparin’s anti-inflammatory effects require glucosamine 6-O-sulfation and are mediated by blockade of L- and P-selectins

Abstract

Research Article

Authors

Lianchun Wang, Jillian R. Brown, Ajit Varki, Jeffrey D. Esko

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Inhibition of selectin-Ig binding to immobilized PAA-SLeX. Inhibition cu...
Inhibition of selectin-Ig binding to immobilized PAA-SLeX. Inhibition curves were generated using OS-heparin (open circles), heparin (open squares), NDS-heparin (asterisks), 2/3DS-heparin (open triangles), N/2/3DS-heparin (filled triangles), 6DS-heparin (filled squares), and CR-heparin (filled circles) (see Methods). Inclusion of a mAb to P-selectin, E-selectin, or EDTA blocked binding by more than 90%. Each point represents the average of duplicate determinations, and the data are representative of three or four separate experiments.