(a) Detection of proliferative T cell response of LNCs from SS model mice to organ-specific autoantigen (JS-1) (*P < 0.001, Student’s t test). No differences were found in OVA (10 μg/ml) and ConA (5 μg/ml) responsiveness. (b) In vitro preventive effect of proliferative T cell response of LNCs, but not spleen cells, to JS-1 by Clik60 in a dose-dependent manner. No inhibitory effects of Clik148 and CA074 were found. Data are expressed as stimulation indices ± SEM. Three experiments from each group were performed at 8 weeks of age, and the mean values of index were statistically significant at *P < 0.05 and **P < 0.01 (Student’s t test). (c) Annexin V/propidium iodide (PI) flow cytometric analysis revealed a small proportion of early apoptotic cells (annexin V–positive, PI-negative) and necrotic cells (annexin V–positive, PI-positive). (d) The inhibitors at 10–7 to 10–4 M concentrations of Clik60 do not block T cell proliferation to Con A (5 μg/ml).