Published November 3, 2008 - More info
This study demonstrates that a CD34–, vascular endothelial cadherin– (VE-cadherin–), AC133+, and fetal liver kinase+ (Flk1+) multipotent adult progenitor cell (MAPC) that copurifies with mesenchymal stem cells from postnatal human bone marrow (BM) is a progenitor for angioblasts. In vitro, MAPCs cultured with VEGF differentiate into CD34+, VE-cadherin+, Flk1+ cells — a phenotype that would be expected for angioblasts. They subsequently differentiate into cells that express endothelial markers, function in vitro as mature endothelial cells, and contribute to neoangiogenesis in vivo during tumor angiogenesis and wound healing. This in vitro model of preangioblast-to-endothelium differentiation should prove very useful in studying commitment to the angioblast and beyond. In vivo, MAPCs can differentiate in response to local cues into endothelial cells that contribute to neoangiogenesis in tumors. Because MAPCs can be expanded in culture without obvious senescence for more than 80 population doublings, they may be an important source of endothelial cells for cellular pro- or anti-angiogenic therapies.
Morayma Reyes, Arkadiusz Dudek, Balkrishna Jahagirdar, Lisa Koodie, Paul H. Marker, Catherine M. Verfaillie
Original citation: J. Clin. Invest.109:337–346 (2002). doi:10.1172/JCI14327.
Citation for this corrigendum: J. Clin. Invest.118:3813 (2008). doi:10.1172/JCI14327C1.
During the preparation of the manuscript, the FACS plot for β2-microglobulin was erroneously duplicated to also represent CD62P in Figure 1B. The legend to Figure 5C was also affected by the error. The correct Figure 1B and legend to Figure 5C appear below.
IL-1α induces expression of HLA-DR, a type of HLA class II antigen, and increases expression of adhesion receptors. MAPC-derived endothelial cells were incubated with or without 75 ng/ml IL-1α in serum-free medium for 24 hours. Cells were stained with Ab’s against HLA class I, HLA class II, β2-microglobulin, vWF, CD31, VCAM, CD62E, CD62P, or control Ab’s and analyzed using FACS. Plots show isotype control IgG staining profile (thin line) versus specific Ab staining profile (thick line). Representative example of three experiments from three donors. Numbers above each plot show MFI for the control IgG staining and the specific Ab staining. Nl, normal; IL-1, induced populations. Note that the histograms for normal β2-microglobulin, HLA class I, and HLA-DR are the same histograms presented in Figure 1B.
The authors regret the error.