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Endothelial C3a receptor mediates vascular inflammation and blood-brain barrier permeability during aging
Nicholas E. Propson, … , Jörg Köhl, Hui Zheng
Nicholas E. Propson, … , Jörg Köhl, Hui Zheng
Published September 29, 2020
Citation Information: J Clin Invest. 2021;131(1):e140966. https://doi.org/10.1172/JCI140966.
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Research Article Aging

Endothelial C3a receptor mediates vascular inflammation and blood-brain barrier permeability during aging

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Abstract

Dysfunction of immune and vascular systems has been implicated in aging and Alzheimer disease; however, their interrelatedness remains poorly understood. The complement pathway is a well-established regulator of innate immunity in the brain. Here, we report robust age-dependent increases in vascular inflammation, peripheral lymphocyte infiltration, and blood-brain barrier (BBB) permeability. These phenotypes were subdued by global inactivation and by endothelial cell–specific ablation of C3ar1. Using an in vitro model of the BBB, we identified intracellular Ca2+ as a downstream effector of C3a/C3aR signaling and a functional mediator of vascular endothelial cadherin junction and barrier integrity. Endothelial C3ar1 inactivation also dampened microglia reactivity and improved hippocampal and cortical volumes in the aging brain, demonstrating a crosstalk between brain vasculature dysfunction and immune cell activation and neurodegeneration. Further, prominent C3aR-dependent vascular inflammation was also observed in a tau-transgenic mouse model. Our studies suggest that heightened C3a/C3aR signaling through endothelial cells promotes vascular inflammation and BBB dysfunction and contributes to overall neuroinflammation in aging and neurodegenerative disease.

Authors

Nicholas E. Propson, Ethan R. Roy, Alexandra Litvinchuk, Jörg Köhl, Hui Zheng

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ISSN: 0021-9738 (print), 1558-8238 (online)

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