(A–D) Representative tracking of animals in the OFT in a (A) CC male (n = 32), (B) CH male (n = 24), (C) CC female (n = 31), and (D) CH female (n = 31). Quantification of ambulatory episodes (E), jump counts (F), rearing events (I), and stereotypic behaviors (J) in CC (circles) versus CH (squares) males at 6 months of age. Quantification of ambulatory episodes (G), jump counts (H), rearing events (K), and stereotypic behaviors (L) in CC (circles) versus CH (squares) females at 6 months of age. (M–T) Chemogenetic activation of young animals at 8 to 10 weeks of age tracked over time after CNO injection at t = 0′ followed by AUC analysis of the second hour (n = 9–15 per diet/genotype/sex, combination of at least 2 different experimental rounds). Vertical counts in males over time (M) and in the second hour (N). Vertical counts in females over time (O) and in the second hour (P). Ambulatory episodes in males over time (Q) and in the second hour (R). Ambulatory episodes in females over time (S) and in the second hour (T). Locomotive behavior in 6-month-old CC versus CH males (U) and females (W) with repeated daily exposure to the OFT. EZM total distance traveled in male (V) and female (X) animals (n = 7–15 per diet/sex/genotype; results indicate a combination of at least 2 separate experiments). *P < 0.05, **P < 0.01, and ***P < 0.001, Student’s t test (E–L) as well as a mixed effects analysis (M, O, Q, and S) or 2-way ANOVA (N, P, R, T, V, X, U, and W with repeated measures).