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RASA1-dependent cellular export of collagen IV controls blood and lymphatic vascular development
Di Chen, … , Philip E. Lapinski, Philip D. King
Di Chen, … , Philip E. Lapinski, Philip D. King
Published June 11, 2019
Citation Information: J Clin Invest. 2019;129(9):3545-3561. https://doi.org/10.1172/JCI124917.
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Research Article Angiogenesis Vascular biology

RASA1-dependent cellular export of collagen IV controls blood and lymphatic vascular development

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Abstract

Combined germline and somatic second-hit inactivating mutations of the RASA1 gene, which encodes a negative regulator of the Ras signaling pathway, cause blood and lymphatic vascular lesions in the human autosomal-dominant vascular disorder capillary malformation–arteriovenous malformation (CM-AVM). How RASA1 mutations in endothelial cells (ECs) result in vascular lesions in CM-AVM is unknown. Here, using different murine models of RASA1 deficiency, we found that RASA1 was essential for the survival of ECs during developmental angiogenesis, in which primitive vascular plexuses are remodeled into hierarchical vascular networks. RASA1 was required for EC survival during developmental angiogenesis, because it was necessary for export of collagen IV from ECs and deposition in vascular basement membranes. In the absence of RASA1, dysregulated Ras/MAPK signal transduction in ECs resulted in impaired folding of collagen IV and its retention in the endoplasmic reticulum (ER), leading to EC death. Remarkably, the chemical chaperone 4-phenylbutyric acid and small-molecule inhibitors of MAPK and 2-oxoglutarate–dependent collagen IV–modifying enzymes rescued ER retention of collagen IV and EC apoptosis and resulted in normal developmental angiogenesis. These findings have important implications for a better understanding of the molecular pathogenesis of CM-AVM and possible means of treatment.

Authors

Di Chen, Joyce M. Teng, Paula E. North, Philip E. Lapinski, Philip D. King

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Figure 3

EC-specific disruption of Rasa1 during developmental angiogenesis results in retention of collagen IV within BECs.

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EC-specific disruption of Rasa1 during developmental angiogenesis result...
TM was administered to littermate Rasa1fl/fl and Rasa1fl/fl Cdh5Ert2Cre embryos at E13.5. Embryos were harvested at E18.5 and skin sections were stained with Hoechst and antibodies against CD31 and collagen IV. Lower-power images are shown in the top rows. Higher-power images of boxed areas are shown below. Note the accumulation of collagen IV within BECs of Rasa1fl/fl Cdh5Ert2Cre embryos (arrows). Scale bars: 30 μm. Magnification: ×1000.

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