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Research Article Free access | 10.1172/JCI119109

An N-linked high-mannose type oligosaccharide, expressed at the major outer membrane protein of Chlamydia trachomatis, mediates attachment and infectivity of the microorganism to HeLa cells.

C Kuo, N Takahashi, A F Swanson, Y Ozeki, and S Hakomori

Department of Pathobiology, University of Washington, Seattle 98195, USA. cckuo@u.washington.edu

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Department of Pathobiology, University of Washington, Seattle 98195, USA. cckuo@u.washington.edu

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Department of Pathobiology, University of Washington, Seattle 98195, USA. cckuo@u.washington.edu

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Department of Pathobiology, University of Washington, Seattle 98195, USA. cckuo@u.washington.edu

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Department of Pathobiology, University of Washington, Seattle 98195, USA. cckuo@u.washington.edu

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Published December 15, 1996 - More info

Published in Volume 98, Issue 12 on December 15, 1996
J Clin Invest. 1996;98(12):2813–2818. https://doi.org/10.1172/JCI119109.
© 1996 The American Society for Clinical Investigation
Published December 15, 1996 - Version history
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Abstract

The structure of the carbohydrate of the 40-kD major outer membrane component of Chlamydia trachomatis and its role in defining infectivity of the organism were investigated. The oligosaccharides were released from the glycoprotein by N-glycanase digestion, coupled to a 2-aminopyridyl residue, and subjected to two-dimensional sugar mapping technique. The major fractions consisted of "high-mannose type" oligosaccharides containing 8-9 mannose residues. Bi- and tri-antennary "complex type" oligosaccharides having terminal galactose were detected as minor components. These oligosaccharides were N-linked and contained no sialic acid. This structural profile is consistent with our previous characterization based on lectin-binding and glycosidase digestion. Functional specificity of identified chlamydial oligosaccharides was analyzed using glycopeptides fractionated from ovalbumin and structurally defined oligosaccharides from other sources. The glycopeptide fraction having high-mannose type oligosaccharide, as compared to those having complex or hybrid-type, showed a stronger inhibitory effect on attachment and infectivity of chlamydial organisms to HeLa cells. Among high-mannose type oligosaccharides, the strongest inhibition was observed with mannose 8 as compared with mannose 6, 7, or 9. These results indicate that a specific high-mannose type oligosaccharide linked to the major outer membrane protein of C. trachomatis mediates attachment and infectivity of the organism to HeLa cells.

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