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Research Article Free access | 10.1172/JCI118475

Cloning, expression, and type II collagenolytic activity of matrix metalloproteinase-13 from human osteoarthritic cartilage.

P G Mitchell, H A Magna, L M Reeves, L L Lopresti-Morrow, S A Yocum, P J Rosner, K F Geoghegan, and J E Hambor

Central Research Division, Pfizer Inc., Groton, Connecticut 06340, USA.

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Central Research Division, Pfizer Inc., Groton, Connecticut 06340, USA.

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Central Research Division, Pfizer Inc., Groton, Connecticut 06340, USA.

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Central Research Division, Pfizer Inc., Groton, Connecticut 06340, USA.

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Central Research Division, Pfizer Inc., Groton, Connecticut 06340, USA.

Find articles by Yocum, S. in: JCI | PubMed | Google Scholar

Central Research Division, Pfizer Inc., Groton, Connecticut 06340, USA.

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Central Research Division, Pfizer Inc., Groton, Connecticut 06340, USA.

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Central Research Division, Pfizer Inc., Groton, Connecticut 06340, USA.

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Published February 1, 1996 - More info

Published in Volume 97, Issue 3 on February 1, 1996
J Clin Invest. 1996;97(3):761–768. https://doi.org/10.1172/JCI118475.
© 1996 The American Society for Clinical Investigation
Published February 1, 1996 - Version history
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Abstract

Proteolysis of triple-helical collagen is an important step in the progression toward irreversible tissue damage in osteoarthritis. Earlier work on the expression of enzymes in cartilage suggested that collagenase-1 (MMP-1) contributes to the process. Degenerate reverse transcription polymerase chain reaction experiments, Northern blot analysis, and direct immunodetection have now provided evidence that collagenase-3 (MMP-13), an enzyme recently cloned from human breast carcinoma, is expressed by chondrocytes in human osteoarthritic cartilage. Variable levels of MMP-13 and MMP-1 in cartilage was significantly induced at both the message and protein levels by interleukin-1 alpha. Recombinant MMP-13 cleaved type II collagen to give characteristic 3/4 and 1/4 fragments; however, MMP-13 turned over type II collagen at least 10 times faster than MMP-1. Experiments with intact type II collagen as well as a synthetic peptide suggested that MMP-13 cleaved type II collagen at the same bond as MMP-1, but this was then followed by a secondary cleavage that removed three amino acids from the 1/4 fragment amino terminus. The expression of MMP-13 in osteoarthritic cartilage and its activity against type II collagen suggest that the enzyme plays a significant role in cartilage collagen degradation, and must consequently form part of a complex target for proposed therapeutic interventions based on collagenase inhibition.

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