Induction of immune unresponsiveness to concordant islet xenografts by intrahepatic preimmunization and transient immunosuppression.

J A Goss; E H Finke; M W Flye; P E Lacy

doi:10.1172/JCI117088

Department of Surgery, Washington University School of Medicine, St. Louis, Missouri 63110.

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Department of Surgery, Washington University School of Medicine, St. Louis, Missouri 63110.

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Department of Surgery, Washington University School of Medicine, St. Louis, Missouri 63110.

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Department of Surgery, Washington University School of Medicine, St. Louis, Missouri 63110.

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Published in Volume 93, Issue 3 on March 1, 1994
J Clin Invest. 1994;93(3):1312–1314. https://doi.org/10.1172/JCI117088.
© 1994 The American Society for Clinical Investigation

Published March 1, 1994 - Version history

Streptozotocin-induced, diabetic mice (C57BL/6) were preimmunized by injecting 25 low temperature, cultured Wistar-Furth (WF) rat islets into the portal vein, and the recipients received one injection of mouse and rat antilymphocyte sera. 3 wk later, fresh WF islets were transplanted under the kidney capsule of the preimmunized recipients, and normoglycemia was maintained in all 13 recipients for 60 d. Removal of the grafts at 60 d returned the mice to a diabetic state. Transplants of fresh WF islets under the kidney capsule without pretreatment of the recipients had a mean survival time of 16.5 +/- 2.5 d. These findings demonstrate that immune unresponsiveness can be achieved across a concordant, islet xenograft barrier within 3 wk after intrahepatic preimmunization with a small number of donor rat islets and transient immunosuppression with antilymphocyte sera.

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