Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • Vascular Malformations (Apr 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
Top
  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal
  • Top
  • Abstract
  • Version history
  • Article usage
  • Citations to this article

Advertisement

Research Article Free access | 10.1172/JCI116572

Regional expression of the platelet-derived growth factor and its receptors in a primate graft model of vessel wall assembly.

L W Kraiss, E W Raines, J N Wilcox, R A Seifert, T B Barrett, T R Kirkman, C E Hart, D F Bowen-Pope, R Ross, and A W Clowes

Department of Surgery, University of Washington, Seattle 98195.

Find articles by Kraiss, L. in: PubMed | Google Scholar

Department of Surgery, University of Washington, Seattle 98195.

Find articles by Raines, E. in: PubMed | Google Scholar

Department of Surgery, University of Washington, Seattle 98195.

Find articles by Wilcox, J. in: PubMed | Google Scholar

Department of Surgery, University of Washington, Seattle 98195.

Find articles by Seifert, R. in: PubMed | Google Scholar

Department of Surgery, University of Washington, Seattle 98195.

Find articles by Barrett, T. in: PubMed | Google Scholar

Department of Surgery, University of Washington, Seattle 98195.

Find articles by Kirkman, T. in: PubMed | Google Scholar

Department of Surgery, University of Washington, Seattle 98195.

Find articles by Hart, C. in: PubMed | Google Scholar

Department of Surgery, University of Washington, Seattle 98195.

Find articles by Bowen-Pope, D. in: PubMed | Google Scholar

Department of Surgery, University of Washington, Seattle 98195.

Find articles by Ross, R. in: PubMed | Google Scholar

Department of Surgery, University of Washington, Seattle 98195.

Find articles by Clowes, A. in: PubMed | Google Scholar

Published July 1, 1993 - More info

Published in Volume 92, Issue 1 on July 1, 1993
J Clin Invest. 1993;92(1):338–348. https://doi.org/10.1172/JCI116572.
© 1993 The American Society for Clinical Investigation
Published July 1, 1993 - Version history
View PDF
Abstract

Healing baboon polytetrafluoroethylene grafts express PDGF mRNA in the neointima. Perfusates of graft segments also contain PDGF-like mitogenic activity. To extend these findings, we studied the expression and regional distribution of the PDGF protein isoforms and their receptors in this prosthetic graft model. By immunohistochemistry, as well as ELISA and Western blot analysis of tissue extracts, both PDGF-A and PDGF-B were identified in macrophages within the interstices of the synthetic material. In contrast, the neointima contained predominantly PDGF-A localized to the endothelial surface and the immediate subjacent smooth muscle cell layers. Tissue extracts of neointima and graft material were mitogenic for baboon aortic smooth muscle cells in culture; nearly all of this proliferative activity was blocked by a neutralizing anti-PDGF antibody. PDGF receptor beta-subunit mRNA and protein were easily detectable in the neointima and graft material. PDGF receptor alpha-subunit mRNA was also observed in the graft matrix and at lower levels in the neointima. This pattern of ligand and receptor expression further implicates locally produced PDGF as a regulator of neointimal smooth muscle cell growth in this model. The coexpression of ligand and receptor in the macrophage-rich matrix also suggests that PDGF may participate in the foreign body response.

Images.

Browse pages

Click on an image below to see the page. View PDF of the complete article

icon of scanned page 338
page 338
icon of scanned page 339
page 339
icon of scanned page 340
page 340
icon of scanned page 341
page 341
icon of scanned page 342
page 342
icon of scanned page 343
page 343
icon of scanned page 344
page 344
icon of scanned page 345
page 345
icon of scanned page 346
page 346
icon of scanned page 347
page 347
icon of scanned page 348
page 348
Version history
  • Version 1 (July 1, 1993): No description

Article tools

  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal

Metrics

  • Article usage
  • Citations to this article

Go to

  • Top
  • Abstract
  • Version history
Advertisement
Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts