Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • Vascular Malformations (Apr 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
Top
  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal
  • Top
  • Abstract
  • Version history
  • Article usage
  • Citations to this article

Advertisement

Research Article Free access | 10.1172/JCI116427

Characterization and distribution of alpha 2-adrenergic receptors in the human intestinal mucosa.

P Valet, J M Senard, J C Devedjian, V Planat, R Salomon, T Voisin, G Drean, A Couvineau, D Daviaud, and C Denis

Institut National de la Santé et de la Recherche Médicale, (INSERM) U317, Institut Louis Bugnard, Centre Hospitalier Universitaire Rangueil, Toulouse, France.

Find articles by Valet, P. in: PubMed | Google Scholar

Institut National de la Santé et de la Recherche Médicale, (INSERM) U317, Institut Louis Bugnard, Centre Hospitalier Universitaire Rangueil, Toulouse, France.

Find articles by Senard, J. in: PubMed | Google Scholar

Institut National de la Santé et de la Recherche Médicale, (INSERM) U317, Institut Louis Bugnard, Centre Hospitalier Universitaire Rangueil, Toulouse, France.

Find articles by Devedjian, J. in: PubMed | Google Scholar

Institut National de la Santé et de la Recherche Médicale, (INSERM) U317, Institut Louis Bugnard, Centre Hospitalier Universitaire Rangueil, Toulouse, France.

Find articles by Planat, V. in: PubMed | Google Scholar

Institut National de la Santé et de la Recherche Médicale, (INSERM) U317, Institut Louis Bugnard, Centre Hospitalier Universitaire Rangueil, Toulouse, France.

Find articles by Salomon, R. in: PubMed | Google Scholar

Institut National de la Santé et de la Recherche Médicale, (INSERM) U317, Institut Louis Bugnard, Centre Hospitalier Universitaire Rangueil, Toulouse, France.

Find articles by Voisin, T. in: PubMed | Google Scholar

Institut National de la Santé et de la Recherche Médicale, (INSERM) U317, Institut Louis Bugnard, Centre Hospitalier Universitaire Rangueil, Toulouse, France.

Find articles by Drean, G. in: PubMed | Google Scholar

Institut National de la Santé et de la Recherche Médicale, (INSERM) U317, Institut Louis Bugnard, Centre Hospitalier Universitaire Rangueil, Toulouse, France.

Find articles by Couvineau, A. in: PubMed | Google Scholar

Institut National de la Santé et de la Recherche Médicale, (INSERM) U317, Institut Louis Bugnard, Centre Hospitalier Universitaire Rangueil, Toulouse, France.

Find articles by Daviaud, D. in: PubMed | Google Scholar

Institut National de la Santé et de la Recherche Médicale, (INSERM) U317, Institut Louis Bugnard, Centre Hospitalier Universitaire Rangueil, Toulouse, France.

Find articles by Denis, C. in: PubMed | Google Scholar

Published May 1, 1993 - More info

Published in Volume 91, Issue 5 on May 1, 1993
J Clin Invest. 1993;91(5):2049–2057. https://doi.org/10.1172/JCI116427.
© 1993 The American Society for Clinical Investigation
Published May 1, 1993 - Version history
View PDF
Abstract

The subtype and the expression of the alpha 2-adrenergic receptor were investigated in the normal mucosa from human intestine by means of radioligand binding, RNase mapping, and measurement of adenylate cyclase activity. The study of the binding of the alpha 2-adrenergic antagonist, [3H]RX821002, to epithelial cell membranes indicated the existence of a single class of noninteracting sites displaying a high affinity for the radioligand (Kd = 1.1 +/- 0.5 nM). The rank order of potency of antagonists to inhibit [3H]RX821002 binding (RX821002 > yohimbine = rauwolscine > phentolamine approximately idazoxan >> chlorpromazine > prazosin) suggested that the receptor is of the alpha 2A subtype. A conclusion which is confirmed by the fact that only alpha 2C10 transcripts were found in the human intestine mucosa. Competition curves with (-)-norepinephrine demonstrated that 60% of the receptor population exhibited high affinity for agonists. This high-affinity state was abolished by the addition of GTP plus Na+ or by prior treatment of the membranes with pertussis toxin indicating it corresponded to G protein-coupled receptors. [32P]ADP-ribosylation and immunoblotting experiments identified two pertussis toxin-sensitive G proteins corresponding to Gi2 and Gi3. The study of the distribution of the receptor indicated that (a) the proximal colon is the intestine segment exhibiting the highest receptor density and (b) the receptor is predominantly expressed in crypts and is preferentially located in the basolateral membrane of the polarized cell. The distribution of the receptor along the crypt-surface axis of the colon mucosa can be correlated with a higher level of alpha 2C10-specific mRNA and a higher efficiency of UK14304 to inhibit adenylate cyclase in crypt cells.

Images.

Browse pages

Click on an image below to see the page. View PDF of the complete article

icon of scanned page 2049
page 2049
icon of scanned page 2050
page 2050
icon of scanned page 2051
page 2051
icon of scanned page 2052
page 2052
icon of scanned page 2053
page 2053
icon of scanned page 2054
page 2054
icon of scanned page 2055
page 2055
icon of scanned page 2056
page 2056
icon of scanned page 2057
page 2057
Version history
  • Version 1 (May 1, 1993): No description

Article tools

  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal

Metrics

  • Article usage
  • Citations to this article

Go to

  • Top
  • Abstract
  • Version history
Advertisement
Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts