Autoantibody reactive with three classes of RNA polymerases in sera from patients with systemic sclerosis.

M Kuwana; J Kaburaki; T Mimori; T Tojo; M Homma

doi:10.1172/JCI116343

Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan.

Find articles by Kuwana, M. in: PubMed | Google Scholar

Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan.

Find articles by Kaburaki, J. in: PubMed | Google Scholar

Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan.

Find articles by Mimori, T. in: PubMed | Google Scholar

Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan.

Find articles by Tojo, T. in: PubMed | Google Scholar

Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan.

Find articles by Homma, M. in: PubMed | Google Scholar

Published in Volume 91, Issue 4 on April 1, 1993
J Clin Invest. 1993;91(4):1399–1404. https://doi.org/10.1172/JCI116343.
© 1993 The American Society for Clinical Investigation

Published April 1, 1993 - Version history

We have identified a novel autoantibody reactive with all three classes of RNA polymerases, well-characterized nuclear enzymes, in sera from patients with systemic sclerosis (SSc). After incubation with [35S]methionine-labeled HeLa cell extracts, 14 of 275 SSc sera immunoprecipitated 12 or 14 proteins with similar molecular weights as those of several subunit proteins of eukaryotic RNA polymerases I, II, and III. Purified IgG from these two types of sera inhibited RNA transcription catalyzed by RNA polymerases I, II, and III in vitro. Immunoblot analysis using RNA polymerase-enriched fraction showed that the majority of these sera reacted with 42- or 25-kD protein. Anti-RNA polymerase antibody was highly specific to SSc, especially to diffuse cutaneous SSc. Clinical features associated with this antibody included a high frequency of heart and kidney involvement and a poor survival rate at 5 yr after first visit. These findings indicate that the autoantibody to three classes of RNA polymerases is a new marker for a unique subset of diffuse cutaneous SSc.

Images.