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Research Article Free access | 10.1172/JCI116147

Binding of type 3 reovirus by a domain of the sigma 1 protein important for hemagglutination leads to infection of murine erythroleukemia cells.

D H Rubin, J D Wetzel, W V Williams, J A Cohen, C Dworkin, and T S Dermody

Department of Medical Research, Veterans Affairs Hospital, Nashville, Tennessee 37212.

Find articles by Rubin, D. in: JCI | PubMed | Google Scholar

Department of Medical Research, Veterans Affairs Hospital, Nashville, Tennessee 37212.

Find articles by Wetzel, J. in: JCI | PubMed | Google Scholar

Department of Medical Research, Veterans Affairs Hospital, Nashville, Tennessee 37212.

Find articles by Williams, W. in: JCI | PubMed | Google Scholar

Department of Medical Research, Veterans Affairs Hospital, Nashville, Tennessee 37212.

Find articles by Cohen, J. in: JCI | PubMed | Google Scholar

Department of Medical Research, Veterans Affairs Hospital, Nashville, Tennessee 37212.

Find articles by Dworkin, C. in: JCI | PubMed | Google Scholar

Department of Medical Research, Veterans Affairs Hospital, Nashville, Tennessee 37212.

Find articles by Dermody, T. in: JCI | PubMed | Google Scholar

Published December 1, 1992 - More info

Published in Volume 90, Issue 6 on December 1, 1992
J Clin Invest. 1992;90(6):2536–2542. https://doi.org/10.1172/JCI116147.
© 1992 The American Society for Clinical Investigation
Published December 1, 1992 - Version history
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Abstract

The recognition of cellular receptors by the mammalian reoviruses is an important determinant of cell and tissue tropism exhibited by reovirus strains of different serotypes. To extend our knowledge of the role of reovirus-receptor interactions in reovirus tropism, we determined whether type 1 and type 3 reovirus strains can infect cells derived from erythrocyte precursors. We found that reovirus type 3 Dearing (T3D), but not type 1 Lang, can grow in murine erythroleukemia (MEL) cells. This difference in growth was investigated by using reassortant viruses and we found that the capacity of T3D to infect MEL cells is determined by the viral cell-attachment protein, sigma 1. In experiments using murine monoclonal antibodies (mAbs) that bind to different sigma 1 regions, we show that T3D binding to MEL cells is inhibited by a mAb that identifies a domain important for hemagglutination (HA). We also determined that type 3 strains that can infect murine L cells but do not produce HA do not infect MEL cells. These results suggest that type 3 reovirus binds to and infects erythrocyte precursor cells via a sigma 1 domain important for HA. Moreover, this study suggests that different domains of some viral cell-attachment proteins are used to initiate productive infections of different types of cells.

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