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Research Article Free access | 10.1172/JCI115986

Epidermal keratinocyte-derived basophil promoting activity. Role of interleukin 3 and soluble CD23.

A H Dalloul, M Arock, C Fourcade, J Y Béranger, P Jaffray, P Debré, and M D Mossalayi

Laboratoire d'Immunologie, Centre National de la Recherche Scientifique URA625, CHU Pitié-Salpêtrière, Paris, France.

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Laboratoire d'Immunologie, Centre National de la Recherche Scientifique URA625, CHU Pitié-Salpêtrière, Paris, France.

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Laboratoire d'Immunologie, Centre National de la Recherche Scientifique URA625, CHU Pitié-Salpêtrière, Paris, France.

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Laboratoire d'Immunologie, Centre National de la Recherche Scientifique URA625, CHU Pitié-Salpêtrière, Paris, France.

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Laboratoire d'Immunologie, Centre National de la Recherche Scientifique URA625, CHU Pitié-Salpêtrière, Paris, France.

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Laboratoire d'Immunologie, Centre National de la Recherche Scientifique URA625, CHU Pitié-Salpêtrière, Paris, France.

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Laboratoire d'Immunologie, Centre National de la Recherche Scientifique URA625, CHU Pitié-Salpêtrière, Paris, France.

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Published October 1, 1992 - More info

Published in Volume 90, Issue 4 on October 1, 1992
J Clin Invest. 1992;90(4):1242–1247. https://doi.org/10.1172/JCI115986.
© 1992 The American Society for Clinical Investigation
Published October 1, 1992 - Version history
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Abstract

Human epidermal keratinocytes (EK) secrete factors able to sustain the proliferation of early myeloid cells and, in particular, the generation of basophils. This activity was previously attributed to IL-3, although no definitive in situ demonstration of this cytokine was provided. In regard to the possible physiological relevance of these data, we investigated herein the nature of EK-derived factors responsible for basophil promotion. Our data show that EK-derived supernatants (EK-sup) contain IL-3 as well as soluble CD23 (sCD23), both known for their colony stimulating activity. Messenger RNA for IL-3 and CD23 were also detected in EK. Blocking experiments using specific neutralizing monoclonal antibodies (mAb) further indicate that EK-derived basophil promoting activity is mainly due to the presence of IL-3 and sCD23 in EK-sup. Furthermore, by contrast to IL-3, sCD23 secretion by EK is cortisone sensitive and highly enhanced by IL-4, suggesting distinct regulatory mechanisms for their production.

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