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Research Article Free access | 10.1172/JCI115844

Human hematopoietic stem cell adherence to cytokines and matrix molecules.

M W Long, R Briddell, A W Walter, E Bruno, and R Hoffman

Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor 48109.

Find articles by Long, M. in: JCI | PubMed | Google Scholar

Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor 48109.

Find articles by Briddell, R. in: JCI | PubMed | Google Scholar

Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor 48109.

Find articles by Walter, A. in: JCI | PubMed | Google Scholar

Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor 48109.

Find articles by Bruno, E. in: JCI | PubMed | Google Scholar

Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor 48109.

Find articles by Hoffman, R. in: JCI | PubMed | Google Scholar

Published July 1, 1992 - More info

Published in Volume 90, Issue 1 on July 1, 1992
J Clin Invest. 1992;90(1):251–255. https://doi.org/10.1172/JCI115844.
© 1992 The American Society for Clinical Investigation
Published July 1, 1992 - Version history
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Abstract

The hematopoietic microenvironment is a complex structure in which stem cells, progenitor cells, stromal cells, growth factors, and extracellular matrix (ECM) molecules each interact to direct the coordinate regulation of blood cell development. While much is known concerning the individual components of this microenvironment, little is understood of the interactions among these various components or, in particular, the nature of those interactions responsible for the regional localization of specific developmental signals. We hypothesized that cytokines act together with ECM molecules to anchor stem cells within the microenvironment, thus modulating their function. In order to analyze matrix-cytokine-stem cell interactions, we developed an ECM model system in which purified stem cell populations and plastic-immobilized individual proteins are used to assess the role of various matrix molecules and/or cytokines in human hematopoietic cell development. Analysis of these interactions revealed that a single ECM protein, thrombospondin, in conjunction with a single cytokine (e.g., c-kit ligand), constitutes a developmental signal that synergistically modulates hematopoietic stem cell function.

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