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Research Article Free access | 10.1172/JCI114519

Differential effects of the various isoforms of platelet-derived growth factor on chemotaxis of fibroblasts, monocytes, and granulocytes.

A Siegbahn, A Hammacher, B Westermark, and C H Heldin

Department of Clinical Chemistry, University Hospital, Uppsala, Sweden.

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Department of Clinical Chemistry, University Hospital, Uppsala, Sweden.

Find articles by Hammacher, A. in: JCI | PubMed | Google Scholar

Department of Clinical Chemistry, University Hospital, Uppsala, Sweden.

Find articles by Westermark, B. in: JCI | PubMed | Google Scholar

Department of Clinical Chemistry, University Hospital, Uppsala, Sweden.

Find articles by Heldin, C. in: JCI | PubMed | Google Scholar

First published March 1, 1990 - More info

Published in Volume 85, Issue 3 (March 1, 1990)
J Clin Invest. 1990;85(3):916–920. doi:10.1172/JCI114519.
Copyright © 1990, The American Society for Clinical Investigation.

Published March 1, 1990
Abstract

The chemotactic activities of three different isoforms of platelet-derived growth factor (PDGF) on fibroblasts, monocytes, and granulocytes of human origin were investigated. PDGF-AB and PDGF-BB induced strong, dose-dependent responses in both fibroblasts and monocytes, whereas PDGF-AA did not stimulate chemotaxis of these cell types. Instead, PDGF-AA inhibited the chemotactic activity of PDGF-AB and PDGF-BB on fibroblasts and monocytes. However, PDGF-AA was not able to block monocyte chemotaxis induced by FMLP. In contrast, in granulocytes, dose-dependent chemotactic responses were obtained with all three isoforms of PDGF. All isoforms gave maximal responses at concentrations between 5 and 20 ng/ml. At higher concentrations the migration was reduced. Reduction and alkylation of the PDGF molecule, which leads to loss of the mitogenic activity, also caused a loss of the chemotactic activities for all three cell types. The data suggest that the various isoforms of PDGF stimulate and inhibit chemotaxis in an isoform- and cell type-specific manner.

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