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Research Article Free access | 10.1172/JCI114437

Regulation of the anti-Sm autoantibody response in systemic lupus erythematosus mice by monoclonal anti-Sm antibodies.

R A Eisenberg, D S Pisetsky, S Y Craven, J P Grudier, M A O'Donnell, and P L Cohen

Department of Medicine, University of North Carolina, Chapel Hill 27599.

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Department of Medicine, University of North Carolina, Chapel Hill 27599.

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Department of Medicine, University of North Carolina, Chapel Hill 27599.

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Department of Medicine, University of North Carolina, Chapel Hill 27599.

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Department of Medicine, University of North Carolina, Chapel Hill 27599.

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Department of Medicine, University of North Carolina, Chapel Hill 27599.

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Published January 1, 1990 - More info

Published in Volume 85, Issue 1 on January 1, 1990
J Clin Invest. 1990;85(1):86–92. https://doi.org/10.1172/JCI114437.
© 1990 The American Society for Clinical Investigation
Published January 1, 1990 - Version history
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Abstract

The administration of certain monoclonal anti-Sm antibodies (2G7, 7.13) induced most MRL/lpr mice to become anti-Sm positive by 5 mo of age, although other anti-Sm monoclonals (Y2, Y12) suppressed the spontaneous response. Positive anti-Sm antibody enhancement occurred efficiently only in MRL/lpr mice and not in other systemic lupus erythematosus mice that have little spontaneous anti-Sm production. The enhancement by anti-Sm antibodies was specific for the anti-Sm response. The mechanism of the passive antibody enhancement was apparently not isotype- or idiotype-related. The fine specificity of the anti-Sm monoclonal antibody may be essential to its enhancing or suppressing effects, since both enhancing monoclonals recognized only the D Sm polypeptide, whereas both suppressing monoclonals saw the D and the B polypeptides. Furthermore, analysis of serial bleeds from unmanipulated MRL mice that developed anti-Sm positivity showed that the D specificity almost always appeared first. We hypothesize, therefore, that those animals in which an anti-Sm response is initiated by D-specific B-cell clones can become serologically positive with the aid of a positive feedback loop. In contrast, animals in which the initial specificity is for both B and D peptides would be prevented from developing a full anti-Sm response.

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