An in vitro human muscle preparation suitable for metabolic studies. Decreased insulin stimulation of glucose transport in muscle from morbidly obese and diabetic subjects.

G L Dohm; E B Tapscott; W J Pories; D J Dabbs; E G Flickinger; D Meelheim; T Fushiki; S M Atkinson; C W Elton; J F Caro

doi:10.1172/JCI113622

Department of Biochemistry, School of Medicine, East Carolina University, Greenville, North Carolina 27858.

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Department of Biochemistry, School of Medicine, East Carolina University, Greenville, North Carolina 27858.

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Department of Biochemistry, School of Medicine, East Carolina University, Greenville, North Carolina 27858.

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Department of Biochemistry, School of Medicine, East Carolina University, Greenville, North Carolina 27858.

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Department of Biochemistry, School of Medicine, East Carolina University, Greenville, North Carolina 27858.

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Department of Biochemistry, School of Medicine, East Carolina University, Greenville, North Carolina 27858.

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Department of Biochemistry, School of Medicine, East Carolina University, Greenville, North Carolina 27858.

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Department of Biochemistry, School of Medicine, East Carolina University, Greenville, North Carolina 27858.

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Department of Biochemistry, School of Medicine, East Carolina University, Greenville, North Carolina 27858.

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Department of Biochemistry, School of Medicine, East Carolina University, Greenville, North Carolina 27858.

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Published in Volume 82, Issue 2 on August 1, 1988
J Clin Invest. 1988;82(2):486–494. https://doi.org/10.1172/JCI113622.
© 1988 The American Society for Clinical Investigation

Published August 1, 1988 - Version history

We have developed an in vitro muscle preparation suitable for metabolic studies with human muscle tissue and have investigated the effects of obesity and non-insulin-dependent diabetes mellitus (NIDDM) on glucose transport. Transport of 3-O-methylglucose and 2-deoxyglucose was stimulated approximately twofold by insulin in muscle from normal nonobese subjects and stimulation occurred in the normal physiological range of insulin concentrations. In contrast to insulin stimulation of 3-O-methylglucose and 2-deoxyglucose transport in muscle from normal, nonobese subjects, tissue from morbidly obese subjects, with or without NIDDM, were not responsive to insulin. Maximal 3-O-methylglucose transport was lower in muscle of obese than nonobese subjects. Morbidly obese patients, with or without NIDDM, have a severe state of insulin resistance in glucose transport. The novel in vitro human skeletal muscle preparation herein described should be useful in investigating the mechanism of this insulin resistance.

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An in vitro human muscle preparation suitable for metabolic studies. Decreased insulin stimulation of glucose transport in muscle from morbidly obese and diabetic subjects.

G L Dohm, E B Tapscott, W J Pories, D J Dabbs, E G Flickinger, D Meelheim, T Fushiki, S M Atkinson, C W Elton, and J F Caro

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An in vitro human muscle preparation suitable for metabolic studies. Decreased insulin stimulation of glucose transport in muscle from morbidly obese and diabetic subjects.

G L Dohm, E B Tapscott, W J Pories, D J Dabbs, E G Flickinger, D Meelheim, T Fushiki, S M Atkinson, C W Elton, and J F Caro

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