Selective suppression of insulin-induced proliferation of cultured human hepatoma cells by somatostatin.

C K Chou; L T Ho; L P Ting; C P Hu; T S Su; W C Chang; C S Suen; M Y Huang; C M Chang

doi:10.1172/JCI112780

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Published in Volume 79, Issue 1 on January 1, 1987
J Clin Invest. 1987;79(1):175–178. https://doi.org/10.1172/JCI112780.
© 1987 The American Society for Clinical Investigation

Published January 1, 1987 - Version history

The effects of somatostatin (SRIF), insulin, and triiodothyronine (T3) on the growth of human hepatoma cells were investigated on the well-differentiated human hepatoma cell line Hep3B. Results showed that both insulin and T3 can stimulate cell growth of serum starved Hep3B cells at physiological concentrations. SRIF alone showed little growth-promoting activity. When added concurrently with insulin, however, SRIF suppressed the insulin-induced cell proliferation in a dose-dependent manner. On the other hand, SRIF had no inhibitory effect on T3-induced cell proliferation. SRIF is labile in the medium, with a half-life of about 2 h during culture incubation. SRIF did not disturb the insulin binding to its surface receptors nor inhibit the insulin-dependent receptor kinase activity of Hep3B cells in vitro. These results suggest that postreceptor regulation may be involved. The selective suppression by SRIF of insulin-induced cell growth provides an unique approach to the study of insulin actions on proliferation of human hepatoma cells.

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Selective suppression of insulin-induced proliferation of cultured human hepatoma cells by somatostatin.

C K Chou, L T Ho, L P Ting, C P Hu, T S Su, W C Chang, C S Suen, M Y Huang, and C M Chang

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Selective suppression of insulin-induced proliferation of cultured human hepatoma cells by somatostatin.

C K Chou, L T Ho, L P Ting, C P Hu, T S Su, W C Chang, C S Suen, M Y Huang, and C M Chang

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