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Modulation of the hydro-osmotic effect of vasopressin on the rabbit cortical collecting tubule by adrenergic agents
Radha K. Krothapalli, … , Harry O. Senekjian, Wadi N. Suki
Radha K. Krothapalli, … , Harry O. Senekjian, Wadi N. Suki
Published July 1, 1983
Citation Information: J Clin Invest. 1983;72(1):287-294. https://doi.org/10.1172/JCI110968.
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Research Article

Modulation of the hydro-osmotic effect of vasopressin on the rabbit cortical collecting tubule by adrenergic agents

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Abstract

The effects of catecholamines on antidiuretic hormone ([Arg8]-vasopressin [AVP])-induced water absorption were evaluated in cortical collecting tubules isolated from the rabbit kidney and perfused in vitro. In the presence of AVP (100 μU/ml), net fluid volume absorption (Jv, nanoliters per minute per millimeter) was 1.14±0.12 and osmotic water permeability coefficient (Pf, X 10-4 centimeters per second) was 217.3±39.9. The addition of the alpha-adrenergic agonist, phenylephrine (PE), in a concentration of 10-6 M resulted in a significant decrease in Jv and Pf to 0.83±0.13 (P < 0.001) and 148.8±41.8 (P < 0.02), respectively. Increasing the concentration of PE to 10-5 M resulted in a further decrease in Jv and Pf to 0.53±0.05 (P < 0.05 vs. PE 10-6 M) and 88.5±9.0 (P 0.05 vs. PE 10-6 M), respectively. In a separate group of tubules, in the presence of AVP (100 μU/ml) and PE (10-5 M), Jv and Pf were 0.35±0.07 and 66.0±17.3, respectively. The addition of the alpha-adrenergic antagonist, phentolamine (PH), in a concentration of 10-6 M resulted in a significant increase in Jv to 1.07±0.19 (P < 0.001) and Pf to 193.3±35.9 (P < 0.005). PH (10-5 M) alone did not significantly affect Jv and Pf in the presence of AVP (100 μU/ml) nor in the presence of 8-bromo adenosine 3',5' cyclic monophosphate (8-BrcAMP). Jv and Pf were 1.20±0.21 and 174.0±25.8, respectively, in the presence of 8-BrcAMP (10-4 M).

Authors

Radha K. Krothapalli, W. Brian Duffy, Harry O. Senekjian, Wadi N. Suki

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