Transport of Apolipoproteins A-I and A-II by Human Thoracic Duct Lymph

David W. Anderson; Ernst J. Schaefer; Thomas J. Bronzert; Frank T. Lindgren; Trudy Forte; Thomas E. Starzl; Gary D. Niblack; Loren A. Zech; H. Bryan Brewer

doi:10.1172/JCI110103

Transport of Apolipoproteins A-I and A-II by Human Thoracic Duct Lymph

David W. Anderson, Ernst J. Schaefer, Thomas J. Bronzert, Frank T. Lindgren, Trudy Forte, Thomas E. Starzl, Gary D. Niblack, Loren A. Zech, and H. Bryan Brewer Jr.

Published March 1, 1981 - More info

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Abstract

The daily transport of human plasma apolipoproteins A-I and A-II, triglyceride, and total cholesterol from the thoracic duct lymph into plasma was measured in two subjects before and three subjects after renal transplantation. Lymph triglyceride transport was ∼83% of the daily ingested fat loads, whereas lymph cholesterol transport was consistently greater than the amount of daily ingested cholesterol. Lymph apolipoprotein transport significantly (P < 0.05) exceeded the predicted apolipoprotein synthesis rate by an average of 659±578 mg/d for apolipoprotein A-I and 109±59 mg/d for apolipoprotein A-II among the five subjects. It is estimated that 22-77% (apolipoprotein A-I) and 28-82% (apolipoprotein A-II) of daily total body apolipoprotein synthesis takes place in the intestine.

Lymph high density lipoprotein particles are mostly high density lipoprotein_2b and high density lipoprotein_2a and have a greater overall relative triglyceride content and a smaller relative cholesteryl ester content when compared with homologous plasma high density lipoproteins. The major quantity of both lymph apolipoprotein A-I (81±8%) and apolipoprotein A-II (90±11%) was found within high density lipoproteins with almost all of the remainder found in chylomicrons and very low density lipoproteins.

The combined results are consistent with a major contribution of the intestine to total body synthesis of apolipoprotein A-I and apolipoprotein A-II. An important role of lymph in returning filtered apolipoprotein to plasma in association with high density lipoproteins is proposed. Accompanying the return of filtered apolipoprotein to the plasma is a probable transformation, both in size and composition, of at least some of the lymph high density lipoprotein_2b and high density lipoprotein_2a particles into high density lipoprotein₃.

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