Lymphocytes were purified from peripheral blood of normal donors and patients with chronic lymphocytic leukemia (CLL) by Ficoll-Hypaque centrifugation. Adenylate cyclase activity, expressed as picomoles [32P]cyclic AMP generated per milligram protein per minute, was 57±4 in normals and 26±4 in CLL patients. Enzyme activity, expressed as picomoles [32P]cyclic AMP generated per 106 lymphocytes per minute, was 2.09±0.19 for normal lymphocytes and 1.10±0.16 for CLL lymphocytes. The differences between normal and CLL peripheral lymphocytes are highly significant (P < 0.001) with either method of calculating activity.
John Mendelsohn, Judith Nordberg