Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • Vascular Malformations (Apr 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
Top
  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal
  • Top
  • Abstract
  • Version history
  • Article usage
  • Citations to this article

Advertisement

Research Article Free access | 10.1172/JCI109403

Effects of Glucose and Parathyroid Hormone on the Renal Handling of Myoinositol by Isolated Perfused Dog Kidneys

B. A. Molitoris, K. A. Hruska, N. Fishman, and W. H. Daughaday

Metabolism Division, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Renal Division, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Find articles by Molitoris, B. in: PubMed | Google Scholar

Metabolism Division, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Renal Division, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Find articles by Hruska, K. in: PubMed | Google Scholar

Metabolism Division, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Renal Division, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Find articles by Fishman, N. in: PubMed | Google Scholar

Metabolism Division, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Renal Division, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Find articles by Daughaday, W. in: PubMed | Google Scholar

Published June 1, 1979 - More info

Published in Volume 63, Issue 6 on June 1, 1979
J Clin Invest. 1979;63(6):1110–1118. https://doi.org/10.1172/JCI109403.
© 1979 The American Society for Clinical Investigation
Published June 1, 1979 - Version history
View PDF
Abstract

The effects of glucose and parathyroid hormone (PTH) on the transport and metabolism of myoinositol (MI) and [2-3H]MI were studied in isolated perfused dog kidneys. Studies during perfusion of kidneys with normal and elevated glucose concentrations demonstrated that under normal conditions the isolated kidney reabsorbed 94.7±0.2% of the filtered MI, and the renal production of 3H-metabolities of MI was 117.9±6% of the filtered MI load. This indicated that entry of MI into tubular cells by reabsorption was not the sole pathway for entry into the pool of MI within the kidney undergoing catabolism. High glucose perfusate decreased MI reabsorption to 68.6±4.7% and thus decreased delivery of [2-3H]MI into the catabolic pool from the reabsorptive pathway. In the high glucose experiments, the rate of [2-3H]MI catabolism exceeded [2-3H]MI reabsorption by the same fraction as in normal glucose experiments, which indicates that high glucose did not affect nonreabsorptive access of MI to the catabolic site.

In contrast to the effects of glucose, PTH administration resulted in an increase in perfusate MI concentration and a decrease in the perfusate [2-3H]MI specific activity. Concomitantly, urinary MI and [2-3H]MI concentrations were increased, again with a decrease in [2-3H]MI specific activity. These results indicate that PTH caused a release of MI into the urine (not the same as decreased MI reabsorption, which would not affect urinary [3H]MI specific activity) and into the perfusate of the isolated kidneys. These effects on MI release were about coincidental with the increase in urinary cyclic 3′,5′-AMP after PTH and preceded the peak phosphaturic effect of PTH. There was no detectable effect of PTH on MI synthesis from glucose as a source of the MI released into the urine and perfusate. However, PTH temporarily halted accumulation of tritiated MI catabolites. There was no effect of inactivated PTH on urinary cyclic 3′,5′-AMP or on MI transport, which indicates that the PTH effect on MI handling was a specific hormonal effect. These studies clarify the renal metabolism of MI, and they demonstrate heretofore unknown effects of PTH on the renal handling and metabolism of MI. The effects of PTH on renal MI metabolism have important implications in renal carbohydrate metabolism and phospholipid turnover.

Browse pages

Click on an image below to see the page. View PDF of the complete article

icon of scanned page 1110
page 1110
icon of scanned page 1111
page 1111
icon of scanned page 1112
page 1112
icon of scanned page 1113
page 1113
icon of scanned page 1114
page 1114
icon of scanned page 1115
page 1115
icon of scanned page 1116
page 1116
icon of scanned page 1117
page 1117
icon of scanned page 1118
page 1118
Version history
  • Version 1 (June 1, 1979): No description

Article tools

  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal

Metrics

  • Article usage
  • Citations to this article

Go to

  • Top
  • Abstract
  • Version history
Advertisement
Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts