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Research Article Free access | 10.1172/JCI109305

Tissue Distribution of Human α1-Microglobulin

Kimiteru Takagi, Kohjin Kin, Yoshihisa Itoh, Tadashi Kawai, Tadashi Kasahara, Toshihiko Shimoda, and Toshio Shikata

Department of Clinical Pathology, Medical Biology, and Parasitology, Jichi Medical School, Tochigi-Ken, Japan, 329-04

Department of Pathology, Nihon University School of Medicine, Tokyo, Japan, 101

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Department of Clinical Pathology, Medical Biology, and Parasitology, Jichi Medical School, Tochigi-Ken, Japan, 329-04

Department of Pathology, Nihon University School of Medicine, Tokyo, Japan, 101

Find articles by Kin, K. in: PubMed | Google Scholar

Department of Clinical Pathology, Medical Biology, and Parasitology, Jichi Medical School, Tochigi-Ken, Japan, 329-04

Department of Pathology, Nihon University School of Medicine, Tokyo, Japan, 101

Find articles by Itoh, Y. in: PubMed | Google Scholar

Department of Clinical Pathology, Medical Biology, and Parasitology, Jichi Medical School, Tochigi-Ken, Japan, 329-04

Department of Pathology, Nihon University School of Medicine, Tokyo, Japan, 101

Find articles by Kawai, T. in: PubMed | Google Scholar

Department of Clinical Pathology, Medical Biology, and Parasitology, Jichi Medical School, Tochigi-Ken, Japan, 329-04

Department of Pathology, Nihon University School of Medicine, Tokyo, Japan, 101

Find articles by Kasahara, T. in: PubMed | Google Scholar

Department of Clinical Pathology, Medical Biology, and Parasitology, Jichi Medical School, Tochigi-Ken, Japan, 329-04

Department of Pathology, Nihon University School of Medicine, Tokyo, Japan, 101

Find articles by Shimoda, T. in: PubMed | Google Scholar

Department of Clinical Pathology, Medical Biology, and Parasitology, Jichi Medical School, Tochigi-Ken, Japan, 329-04

Department of Pathology, Nihon University School of Medicine, Tokyo, Japan, 101

Find articles by Shikata, T. in: PubMed | Google Scholar

Published February 1, 1979 - More info

Published in Volume 63, Issue 2 on February 1, 1979
J Clin Invest. 1979;63(2):318–325. https://doi.org/10.1172/JCI109305.
© 1979 The American Society for Clinical Investigation
Published February 1, 1979 - Version history
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Abstract

Human α1-microglobulin was isolated from the urine of patients with tubular proteinuria, and its molecular weight was established by sodium dodecyl sulfate-polyacrylamide gel electrophoresis at 33,000 daltons. The carbohydrate content was 21.7%. Anti-α1-microglobulin serum was prepared and observed to react monospecifically in gel diffusion to purified α1-microglobulin, as well as to normal human serum and urine. Sera from the domestic chicken, mouse, rat, rabbit, dog, calf, cow, goat, sheep, and horse, however, did not react to anti-α1-microglobulin serum in immunodiffusion. The lymphocyte culture supernate was found to contain α1-microglobulin. Both thymus-derived(T)- and bone marrow-derived(B)-lymphocyte culture media clearly displayed a specific precipitin line against anti-α1-microglobulin serum when tested with the Ouchterlony immunodiffusion method. The tissue distribution of α1-microglobulin was studied under immunofluorescence, and a positive staining was recognized on the lymphocyte surface. Identical staining patterns were noted on both T and B lymphocytes, though B lymphocytes took a more intense stain. It would thus seem quite possible that lymphocytes are the primary source of α1-microglobulin and that this is filtered through the glomerular basement membrane and partly reabsorbed by the renal tubules. This, then, would suggest the possibility that α1-microglobulin shares some immunological role in vivo with lymphocytes and(or) is one of the membrane proteins of lymphocytes.

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