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Usage Information

Immunologic and Nonimmunologic Generation of Superoxide from Mast Cells and Basophils
William R. Henderson, Michael Kaliner
William R. Henderson, Michael Kaliner
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Research Article

Immunologic and Nonimmunologic Generation of Superoxide from Mast Cells and Basophils

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Abstract

Mediator release from rat peritoneal and human lung mast cells as well as human leukemic basophils was examined to determine whether super-oxide (O−2) was concomitantly generated. Immunologic or nonimmunologic stimulation of each preparation induced parallel release of histamine and O−2 within 2 min. O−2 production was quantitated by superoxide dismutase (SOD)-inhibitable chemiluminescence and cytochrome c reduction. SOD was detected in basophil and mast cell lysates and was also released by rat mast cells stimulated by anti-IgE. Secretory granules isolated from purified rat mast cells released histamine, O−2, and SOD upon exposure to cations. Thus, both superoxide radicals and SOD may play a role in host defenses involved in immediate hypersensitivity reactions.

Authors

William R. Henderson, Michael Kaliner

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Usage data is cumulative from September 2025 through September 2026.

Usage JCI PMC
Text version 398 9
PDF 172 7
Figure 0 1
Scanned page 912 1
Citation downloads 226 0
Totals 1,708 18
Total Views 1,726
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ISSN: 0021-9738 (print), 1558-8238 (online)

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