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Research Article Free access | 10.1172/JCI107376

Lymphocyte Transformation during Dinitrochlorobenzene Contact Sensitization. AN IN VITRO AND IN VIVO EVALUATION OF THE PRIMARY IMMUNE RESPONSE IN MAN

A. Edgar Miller and William R. Levis

1Dermatology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014

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1Dermatology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014

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Published August 1, 1973 - More info

Published in Volume 52, Issue 8 on August 1, 1973
J Clin Invest. 1973;52(8):1925–1930. https://doi.org/10.1172/JCI107376.
© 1973 The American Society for Clinical Investigation
Published August 1, 1973 - Version history
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Abstract

An evaluation of cell-mediated immunity in man is described that combines the advantages of an in vitro technique, lymphocyte transformation, with the use of contact sensitization to a primary immunogen, dinitrochlorobenzene (DNCB). DNCB, when coupled to autologous or allogeneic peripheral blood leukocytes, forms a complex, DNCB-antigen, that induces lymphocyte transformation specifically in leukocyte cultures from subjects sensitized to DNCB. Sequential studies of lymphocyte transformation to DNCB-antigen show that specifically reactive lymphocytes are first detected at about 10 days after in vivo application of a sensitizing dose of DNCB and reach a peak at about 14-21 days.

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