Dynamics of Insulin Release and Microtubular-Microfilamentous System. I. EFFECT OF CYTOCHALASIN B

E. Van Obberghen; G. Somers; G. Devis; G. D. Vaughan; F. Malaisse-Lagae; L. Orci; W. J. Malaisse

doi:10.1172/JCI107269

^{Laboratory of Experimental Medicine, Vrije Universiteit Brussel and Université Libre de Bruxelles, Brussels, Belgium}

^{Institute of Histology and Biochimie Clinique, Université de Genève, Geneva, Switzerland}

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^{Laboratory of Experimental Medicine, Vrije Universiteit Brussel and Université Libre de Bruxelles, Brussels, Belgium}

^{Institute of Histology and Biochimie Clinique, Université de Genève, Geneva, Switzerland}

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^{Laboratory of Experimental Medicine, Vrije Universiteit Brussel and Université Libre de Bruxelles, Brussels, Belgium}

^{Institute of Histology and Biochimie Clinique, Université de Genève, Geneva, Switzerland}

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^{Laboratory of Experimental Medicine, Vrije Universiteit Brussel and Université Libre de Bruxelles, Brussels, Belgium}

^{Institute of Histology and Biochimie Clinique, Université de Genève, Geneva, Switzerland}

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^{Laboratory of Experimental Medicine, Vrije Universiteit Brussel and Université Libre de Bruxelles, Brussels, Belgium}

^{Institute of Histology and Biochimie Clinique, Université de Genève, Geneva, Switzerland}

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^{Laboratory of Experimental Medicine, Vrije Universiteit Brussel and Université Libre de Bruxelles, Brussels, Belgium}

^{Institute of Histology and Biochimie Clinique, Université de Genève, Geneva, Switzerland}

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^{Laboratory of Experimental Medicine, Vrije Universiteit Brussel and Université Libre de Bruxelles, Brussels, Belgium}

^{Institute of Histology and Biochimie Clinique, Université de Genève, Geneva, Switzerland}

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Published in Volume 52, Issue 5 on May 1, 1973
J Clin Invest. 1973;52(5):1041–1051. https://doi.org/10.1172/JCI107269.
© 1973 The American Society for Clinical Investigation

Published May 1, 1973 - Version history

In order to assess the participation of the microfilamentous cell web in the multiphasic response of the pancreatic beta cell, the effect of cytochalasin B upon both glucose- and sulfonylurea-induced insulin release was investigated in the perfused isolated pancreas. Cytochalasin B failed to affect the basal rate of insulin release, but enhanced the initial and later phases of insulin secretion in response to either glucose or gliclazide. In addition, cytochalasin B lowered the threshold concentration for the stimulant action of glucose upon insulin release. Ultrastructural studies supported the concept of a specific interaction of cytochalasin B with the microfilamentous cell web of the beta cell. It is concluded that the integrity of such a structure is equally important for both the initial and later secretory responses of the beta cell to various insulinotropic agents.

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