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Concise Publication Free access | 10.1172/JCI107124
1Cancer Research Institute and the Department of Medicine, University of California School of Medicine, San Francisco, California 94122
Find articles by Golde, D. in: JCI | PubMed | Google Scholar
1Cancer Research Institute and the Department of Medicine, University of California School of Medicine, San Francisco, California 94122
Find articles by Cline, M. in: JCI | PubMed | Google Scholar
Published November 1, 1972 - More info
Bone marrow colony formation in soft gel culture may be stimulated by substances elaborated by human peripheral blood leukocytes. In order to determine the cell type responsible for colony stimulation, peripheral leukocytes were separated by Ficoll-Hypaque gradients and differential glass adhesion. Morphologic, histochemical, and functional criteria were applied to determine the purity of the monocyte, lymphocyte, and neutrophil fractions.
Using these cells as feeder layers and as a source of conditioned medium, evidence was obtained that the monocyte is the colony-stimulating cell of human peripheral blood. Activity greater than that of mixed white cells was obtained with monocyte underlayers, and only monocyte- and macrophage-conditioned media were shown to have significant colony-stimulating activity.
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