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Concise Publication Free access | 10.1172/JCI107124

Identification of the colony-stimulating cell in human peripheral blood

David W. Golde and Martin J. Cline

1Cancer Research Institute and the Department of Medicine, University of California School of Medicine, San Francisco, California 94122

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1Cancer Research Institute and the Department of Medicine, University of California School of Medicine, San Francisco, California 94122

Find articles by Cline, M. in: PubMed | Google Scholar

Published November 1, 1972 - More info

Published in Volume 51, Issue 11 on November 1, 1972
J Clin Invest. 1972;51(11):2981–2983. https://doi.org/10.1172/JCI107124.
© 1972 The American Society for Clinical Investigation
Published November 1, 1972 - Version history
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Abstract

Bone marrow colony formation in soft gel culture may be stimulated by substances elaborated by human peripheral blood leukocytes. In order to determine the cell type responsible for colony stimulation, peripheral leukocytes were separated by Ficoll-Hypaque gradients and differential glass adhesion. Morphologic, histochemical, and functional criteria were applied to determine the purity of the monocyte, lymphocyte, and neutrophil fractions.

Using these cells as feeder layers and as a source of conditioned medium, evidence was obtained that the monocyte is the colony-stimulating cell of human peripheral blood. Activity greater than that of mixed white cells was obtained with monocyte underlayers, and only monocyte- and macrophage-conditioned media were shown to have significant colony-stimulating activity.

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