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Research Article Free access | 10.1172/JCI106999

Receptors for complement and immunoglobulin on human leukemic cells and human lymphoblastoid cell lines

Ethan M. Shevach, Ronald Herberman, Michael M. Frank, and Ira Green

Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014

Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014

Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014

Find articles by Shevach, E. in: JCI | PubMed | Google Scholar

Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014

Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014

Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014

Find articles by Herberman, R. in: JCI | PubMed | Google Scholar

Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014

Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014

Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014

Find articles by Frank, M. in: JCI | PubMed | Google Scholar

Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014

Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014

Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014

Find articles by Green, I. in: JCI | PubMed | Google Scholar

Published August 1, 1972 - More info

Published in Volume 51, Issue 8 on August 1, 1972
J Clin Invest. 1972;51(8):1933–1938. https://doi.org/10.1172/JCI106999.
© 1972 The American Society for Clinical Investigation
Published August 1, 1972 - Version history
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Abstract

The bone marrow-derived (B) lymphocyte can be identified by the presence of easily detectable surface immunoglobulin and a receptor for antigen-antibody-complement complexes (EAC′). Monocytes and macrophages also bear a receptor for EAC′ and in addition possess a receptor for red cell-IgG complexes (EA). Thymus-derived (T) lymphocytes bear neither of these receptors. The cells of 15 patients with leukemia and 19 human lymphoblastoid cell lines were examined for the presence of the EAC′ and EA receptors. Of the human leukemias studied, only the cells from the patients with chronic lymphatic leukemia (CLL) possess the EAC′ receptor. The EA receptor could not be demonstrated on CLL cells; hence, CLL cells bear the lymphocyte EAC′ receptor and by this criteria represent B lymphocytes. 12/19 of the cell lines studied could be classified as B lymphocytes by the presence of the EAC′ receptor and absence of the EA receptor. 2/19 cell lines possessed both the EAC′ and EA receptors and thus resemble the monocyte. 5/19 cell lines had no detectable receptor for EAC′ or EA. The approach presented in this study for the classification of leukemias and cell lines as to their B lymphocyte, T lymphocyte, or monocyte origin may have useful diagnostic and therapeutic implications.

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