The appearance of lecithin-<sup>32</sup>P, synthesized from lysolecithin-<sup>32</sup>P, in phagosomes of polymorphonuclear leukocytes

P. Elsbach; P. Patriarca; P. Pettis; T. P. Stossel; R. J. Mason; M. Vaughan

doi:10.1172/JCI106994

^{Department of Medicine, New York University School of Medicine, New York 10016}

^{The Molecular Disease Branch, National Heart and Lung Institute, National Institutes of Health, Bethesda, Maryland 20014}

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^{Department of Medicine, New York University School of Medicine, New York 10016}

^{The Molecular Disease Branch, National Heart and Lung Institute, National Institutes of Health, Bethesda, Maryland 20014}

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^{Department of Medicine, New York University School of Medicine, New York 10016}

^{The Molecular Disease Branch, National Heart and Lung Institute, National Institutes of Health, Bethesda, Maryland 20014}

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^{Department of Medicine, New York University School of Medicine, New York 10016}

^{The Molecular Disease Branch, National Heart and Lung Institute, National Institutes of Health, Bethesda, Maryland 20014}

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^{Department of Medicine, New York University School of Medicine, New York 10016}

^{The Molecular Disease Branch, National Heart and Lung Institute, National Institutes of Health, Bethesda, Maryland 20014}

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^{Department of Medicine, New York University School of Medicine, New York 10016}

^{The Molecular Disease Branch, National Heart and Lung Institute, National Institutes of Health, Bethesda, Maryland 20014}

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Published in Volume 51, Issue 7 on July 1, 1972
J Clin Invest. 1972;51(7):1910–1914. https://doi.org/10.1172/JCI106994.
© 1972 The American Society for Clinical Investigation

Published July 1, 1972 - Version history

Rabbit polymorphonuclear leukocytes ingesting paraffin oil particles stabilized with albumin, converted more lysolecithin-³²P (added to the medium as an albumin complex) to cellular lecithin than did control cells.

Almost all of the increment in leukocyte lecithin-³²P is found in association with the isolated phagocytic vacuoles.

About half of lecithin-³²P of granulocytes incubated first with lysolecithin-³²P and then reincubated with paraffin particles in a nonradioactive medium is transferred from a sedimentable (presumably membrane) fraction to the phagosomes. Isolated phagosomes or granules by themselves are capable of acylating lysolecithin. The main source of lysolecithin-³²P for synthesis of cellular lecithin-³²P, however, appears to be extracellular rather than lysolecithin-³²P within the cytoplasm or the phagocytic vacuole. We interpret our findings therefore as indicating that lecithin-³²P in the phagosomes derives chiefly from the outer membrane.