Preparation and Properties of Thyroxine-Binding Alpha Globulin (TBG)

Kenneth Sterling; Satoshi Hamada; Yoshihiro Takemura; Milton A. Brenner; Edward S. Newman; Mitsuo Inada

doi:10.1172/JCI106665

Amendment history:

Correction (October 1971)

Preparation and Properties of Thyroxine-Binding Alpha Globulin (TBG)

Kenneth Sterling, Satoshi Hamada, Yoshihiro Takemura, Milton A. Brenner, Edward S. Newman, and Mitsuo Inada

Published August 1, 1971 - More info

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Abstract

Thyroxine-binding alpha globulin (TBG) in human serum was isolated from Cohn fractions IV-5,6 and IV-4 by (1) chromatography on carboxymethyl (CM) cellulose, (2) gel filtration on Sephadex G-200, (3) chromatography on diethylaminoethyl-Sephadex, (4) a novel procedure of “double-gel” electrophoresis, and (5) preparative polyacrylamide gel electrophoresis. The protein was homogeneous by analytical disc gel electrophoresis, immunoelectrophoresis, and ultracentrifugal analyses (sedimentation velocity and sedimentation equilibrium), and after addition of thyroxine-¹²⁵I showed a constant specific radioactivity on polyacrylamide electrophoresis. The sedimentation and diffusion coefficients were s_{20, w}, 3.0 × 10⁻¹³ sec, and D_{20, w}, 8.05 × 10⁻⁷ cm²·sec⁻¹, and the molecular weight obtained by sedimentation equilibrium was 36,500. Gel filtration studies on Sephadex G-200 demonstrated that the protein had the same elution volume as that of native TBG in serum, apparently excluding the possibility of a subunit of the native protein. Chemical composition was ascertained by amino acid and carbohydrate analyses. The maximal thyroxine (T4)-binding capacity measured by reverse flow paper electrophoresis was 15,000 μg per g of protein, representing more than 2100 times that of the starting material, or about 5000 times that of whole serum. Based on the molecular weight obtained, the TBG preparation could bind 0.7 mole T4 per mole of protein, suggesting a single binding site. The association constant for T4 was estimated to be of the order of 10¹⁰ by competitive binding studies employing TBG and T4-binding prealbumin (TBPA).