Materials Indistinguishable from Iodotyrosines in Normal Human Serum and Human Serum Albumin

Henry Weinert; Hideo Masui; Ildiko Radichevich; Sidney C. Werner

doi:10.1172/JCI105619

Department of Medicine, Columbia University College of Physicians [unk] Surgeons, and the Presbyterian Hospital, New York, N. Y.

†

Address requests for reprints to Dr. Sidney C. Werner, Dept. of Medicine, Columbia University College of Physicians & Surgeons, 630 W. 168th St., New York, N. Y. 10032.

Submitted for publication December 19, 1966; accepted April 13, 1967.

Aided by grants AM-00008 (C14-18 inc.) from the National Institute of Arthritis and Metabolic Diseases.

Find articles by Weinert, H. in: JCI | PubMed | Google Scholar

Department of Medicine, Columbia University College of Physicians [unk] Surgeons, and the Presbyterian Hospital, New York, N. Y.

†

Address requests for reprints to Dr. Sidney C. Werner, Dept. of Medicine, Columbia University College of Physicians & Surgeons, 630 W. 168th St., New York, N. Y. 10032.

Submitted for publication December 19, 1966; accepted April 13, 1967.

Aided by grants AM-00008 (C14-18 inc.) from the National Institute of Arthritis and Metabolic Diseases.

Find articles by Masui, H. in: JCI | PubMed | Google Scholar

Department of Medicine, Columbia University College of Physicians [unk] Surgeons, and the Presbyterian Hospital, New York, N. Y.

†

Address requests for reprints to Dr. Sidney C. Werner, Dept. of Medicine, Columbia University College of Physicians & Surgeons, 630 W. 168th St., New York, N. Y. 10032.

Submitted for publication December 19, 1966; accepted April 13, 1967.

Aided by grants AM-00008 (C14-18 inc.) from the National Institute of Arthritis and Metabolic Diseases.

Find articles by Radichevich, I. in: JCI | PubMed | Google Scholar

Department of Medicine, Columbia University College of Physicians [unk] Surgeons, and the Presbyterian Hospital, New York, N. Y.

†

Address requests for reprints to Dr. Sidney C. Werner, Dept. of Medicine, Columbia University College of Physicians & Surgeons, 630 W. 168th St., New York, N. Y. 10032.

Submitted for publication December 19, 1966; accepted April 13, 1967.

Aided by grants AM-00008 (C14-18 inc.) from the National Institute of Arthritis and Metabolic Diseases.

Find articles by Werner, S. in: JCI | PubMed | Google Scholar

Published in Volume 46, Issue 7 on July 1, 1967
J Clin Invest. 1967;46(7):1264–1274. https://doi.org/10.1172/JCI105619.
© 1967 The American Society for Clinical Investigation

Published July 1, 1967 - Version history

Materials indistinguishable from authentic mono- and diiodotyrosines were identified in extracts of normal human serum as well as in extracts of purified human serum albumin. These materials were not found in association with the other serum proteins. Identification of MIT and DIT was made by a technique using rechromatography to constant specific activity, as well as by the Barker wet ash distillation method, which established the compounds in question as being iodinated ones. By two different extraction and chromatographic methods we estimated the amounts of both MIT and DIT present in normal human serum or albumin; the estimates were in good agreement. These compounds together constituted between 19% and 25% of the extractable serum iodine.

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