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Research Article Free access | 10.1172/JCI105563
Department of Medicine, University of Wisconsin Medical School, Madison, Wis.
†Postdoctoral research fellow.
‡Research fellow of the Netherlands Organization for the Advancement of Pure Research (ZWO).
§Address requests for reprints to Dr. Robert F. Schilling, Dept. of Medicine, University of Wisconsin, 1300 University Ave., Madison, Wis. 53706.
*Submitted for publication September 20, 1966; accepted December 22, 1966.
Supported by research and training grants from the National Institutes of Health.
Find articles by Schade, S. in: JCI | PubMed | Google Scholar
Department of Medicine, University of Wisconsin Medical School, Madison, Wis.
†Postdoctoral research fellow.
‡Research fellow of the Netherlands Organization for the Advancement of Pure Research (ZWO).
§Address requests for reprints to Dr. Robert F. Schilling, Dept. of Medicine, University of Wisconsin, 1300 University Ave., Madison, Wis. 53706.
*Submitted for publication September 20, 1966; accepted December 22, 1966.
Supported by research and training grants from the National Institutes of Health.
Find articles by Abels, J. in: JCI | PubMed | Google Scholar
Department of Medicine, University of Wisconsin Medical School, Madison, Wis.
†Postdoctoral research fellow.
‡Research fellow of the Netherlands Organization for the Advancement of Pure Research (ZWO).
§Address requests for reprints to Dr. Robert F. Schilling, Dept. of Medicine, University of Wisconsin, 1300 University Ave., Madison, Wis. 53706.
*Submitted for publication September 20, 1966; accepted December 22, 1966.
Supported by research and training grants from the National Institutes of Health.
Find articles by Schilling, R. in: JCI | PubMed | Google Scholar
Department of Medicine, University of Wisconsin Medical School, Madison, Wis.
†Postdoctoral research fellow.
‡Research fellow of the Netherlands Organization for the Advancement of Pure Research (ZWO).
§Address requests for reprints to Dr. Robert F. Schilling, Dept. of Medicine, University of Wisconsin, 1300 University Ave., Madison, Wis. 53706.
*Submitted for publication September 20, 1966; accepted December 22, 1966.
Supported by research and training grants from the National Institutes of Health.
Find articles by Feick, P. in: JCI | PubMed | Google Scholar
Department of Medicine, University of Wisconsin Medical School, Madison, Wis.
†Postdoctoral research fellow.
‡Research fellow of the Netherlands Organization for the Advancement of Pure Research (ZWO).
§Address requests for reprints to Dr. Robert F. Schilling, Dept. of Medicine, University of Wisconsin, 1300 University Ave., Madison, Wis. 53706.
*Submitted for publication September 20, 1966; accepted December 22, 1966.
Supported by research and training grants from the National Institutes of Health.
Find articles by Muckerheide, M. in: JCI | PubMed | Google Scholar
Published April 1, 1967 - More info
Sera from a group of 79 patients with pernicious anemia were studied for the presence of antibody to intrinsic factor. Two general types of antibody activity were found, and it was possible to distinguish three groups of pernicious anemia sera on the basis of their content of these types.
Type I antibody blocks the binding of radioactive vitamin B12 to intrinsic factor when added to intrinsic factor before the B12; it is not detected on intrinsic factor when added after B12. This antibody blocks intrinsic factor-mediated B12 absorption in vivo when mixed in the sequence intrinsic factor + antibody I + B12, but not when mixed in the sequence intrinsic factor + B12 + antibody I.
Type II antibody reacts with intrinsic factor when B12 is attached. This antibody prevents the absorption of B12 from intrinsic factor in pernicious anemia patients when mixed in the sequence intrinsic factor + B12 + antibody II, and is thereby distinguished from antibody I.