Submit a comment
Citation Information: J Clin Invest. 1985;76(2):878-881. https://doi.org/10.1172/JCI112046.
Abstract
L6 cells have been investigated as a potential tissue culture model for the study of thyroid hormone effects on skeletal muscle metabolism. Differentiated L6 myotubes contained high-affinity triiodothyronine (T3) receptors with a Kd of 3 X 10(-10) M and a maximal binding capacity of 24 fmol T3/100 micrograms DNA. Undifferentiated cells contained receptors with the same Kd, but the binding capacity was reduced by at least a factor of three. Sarcoplasmic reticulum vesicle calcium pumping was demonstrated in L6 cell homogenates. The Vmax for calcium pumping was increased 2.5-fold when T3 was present in the culture medium, but the Kd was unchanged. L6 cells contained high affinity thyroid hormone receptors and were thyroid hormone responsive. These cells may be useful as a tissue culture model for studying the effects of thyroid hormones on skeletal muscle metabolism. In addition, the increase in T3 receptor number with the differentiated state suggests this as a model system for studying regulation of T3 receptor number and the role of T3 in the induction or maintenance of the differentiated state.
Authors
R J Koenig, R J Smith
Guidelines
The Editorial Board will only consider comments that are deemed relevant and of interest to readers. The Journal will not post data that have not been subjected to peer review; or a comment that is essentially a reiteration of another comment.
- Comments appear on the Journal’s website and are linked from the original article’s web page.
- Authors are notified by email if their comments are posted.
- The Journal reserves the right to edit comments for length and clarity.
- No appeals will be considered.
- Comments are not indexed in PubMed.
Specific requirements
- Maximum length, 400 words
- Entered as plain text or HTML
- Author’s name and email address, to be posted with the comment
- Declaration of all potential conflicts of interest (even if these are not ultimately posted); see the Journal’s conflict-of-interest policy
- Comments may not include figures
Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)