Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Pancreatic Cancer (Jul 2025)
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
Top
  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal
  • Top
  • Abstract
  • Version history
  • Article usage
  • Citations to this article

Advertisement

Research Article Free access | 10.1172/JCI118288

Oxidatively modified LDL contains phospholipids with platelet-activating factor-like activity and stimulates the growth of smooth muscle cells.

J M Heery, M Kozak, D M Stafforini, D A Jones, G A Zimmerman, T M McIntyre, and S M Prescott

Nora Eccles Harrison Cardiovascular Research & Training Institute, University of Utah, Salt Lake City 84112, USA.

Find articles by Heery, J. in: PubMed | Google Scholar

Nora Eccles Harrison Cardiovascular Research & Training Institute, University of Utah, Salt Lake City 84112, USA.

Find articles by Kozak, M. in: PubMed | Google Scholar

Nora Eccles Harrison Cardiovascular Research & Training Institute, University of Utah, Salt Lake City 84112, USA.

Find articles by Stafforini, D. in: PubMed | Google Scholar

Nora Eccles Harrison Cardiovascular Research & Training Institute, University of Utah, Salt Lake City 84112, USA.

Find articles by Jones, D. in: PubMed | Google Scholar

Nora Eccles Harrison Cardiovascular Research & Training Institute, University of Utah, Salt Lake City 84112, USA.

Find articles by Zimmerman, G. in: PubMed | Google Scholar

Nora Eccles Harrison Cardiovascular Research & Training Institute, University of Utah, Salt Lake City 84112, USA.

Find articles by McIntyre, T. in: PubMed | Google Scholar

Nora Eccles Harrison Cardiovascular Research & Training Institute, University of Utah, Salt Lake City 84112, USA.

Find articles by Prescott, S. in: PubMed | Google Scholar

Published November 1, 1995 - More info

Published in Volume 96, Issue 5 on November 1, 1995
J Clin Invest. 1995;96(5):2322–2330. https://doi.org/10.1172/JCI118288.
© 1995 The American Society for Clinical Investigation
Published November 1, 1995 - Version history
View PDF
Abstract

Oxidative modification of lipoproteins is believed to be important in the genesis of atherosclerosis. We established cultures of smooth muscle cells (SMC) and exposed them to native LDL or oxidized LDL. Oxidized LDL, but not native LDL, was mitogenic as measured by incorporation of [3H]-thymidine into DNA. This effect was concentration dependent, averaged 288% of control, and was blocked by a platelet-activating factor (PAF) receptor antagonist. We hypothesized that phospholipids with PAF-like activity were generated during the oxidation of LDL. To test this hypothesis we extracted phospholipids from copper-oxidized LDL and assayed for PAF-like activity. Phospholipids extracted from oxidized LDL and purified by HPLC induced neutrophil adhesion equivalent to PAF (10 nM) and were mitogenic for smooth muscle cells. These effects were not seen with phospholipids extracted from native LDL and were blocked by two structurally different, competitive antagonists of the PAF receptor. The effects of these lipids were also abolished by pretreating them with PAF acetylhydrolase. Finally, we used Chinese hamster ovary cells that had seen stably transfected with a cDNA for the PAF receptor to confirm that phospholipids from oxidized LDL act via this receptor. We found that PAF (control) and the oxidized phospholipids each induced release of arachidonic acid from the transfected cells, but had no effect on wildtype Chinese hamster ovary cells, which lack the PAF receptor. This effect was also blocked by a PAF receptor antagonist. Thus, phospholipids generated during oxidative modification of LDL may participate in atherosclerosis by stimulating SMC proliferation and leukocyte activation.

Images.

Browse pages

Click on an image below to see the page. View PDF of the complete article

icon of scanned page 2322
page 2322
icon of scanned page 2323
page 2323
icon of scanned page 2324
page 2324
icon of scanned page 2325
page 2325
icon of scanned page 2326
page 2326
icon of scanned page 2327
page 2327
icon of scanned page 2328
page 2328
icon of scanned page 2329
page 2329
icon of scanned page 2330
page 2330
Version history
  • Version 1 (November 1, 1995): No description

Article tools

  • View PDF
  • Download citation information
  • Send a comment
  • Terms of use
  • Standard abbreviations
  • Need help? Email the journal

Metrics

  • Article usage
  • Citations to this article

Go to

  • Top
  • Abstract
  • Version history
Advertisement
Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts