Antigen CD34+ marrow cells engraft lethally irradiated baboons.

R J Berenson; R G Andrews; W I Bensinger; D Kalamasz; G Knitter; C D Buckner; I D Bernstein

doi:10.1172/JCI113409

Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.

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Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.

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Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.

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Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.

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Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.

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Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.

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Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.

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Published in Volume 81, Issue 3 on March 1, 1988
J Clin Invest. 1988;81(3):951–955. https://doi.org/10.1172/JCI113409.
© 1988 The American Society for Clinical Investigation

Published March 1, 1988 - Version history

The CD34 antigen is present on 1-4% of human marrow cells including virtually all hematopoietic progenitors detected by in vitro assays. Since the anti-CD34 monoclonal antibody 12-8 reacts with a similar marrow population in baboons, it was possible to test whether this antigen is expressed by stem cells responsible for hematopoietic reconstitution in vivo. CD34+ cells were enriched from marrows of five baboons using avidin-biotin immunoadsorption. After lethal irradiation, the five animals were given 15-27 X 10(6) autologous marrow cells (3.2-4.4 X 10(6) cells/kg) containing 65-91% CD34+ cells. All animals achieved granulocyte counts greater than 1,000/mm3 and platelet counts greater than 20 X 10(3)/mm3 by 13-24 d posttransplant and subsequently developed normal peripheral blood counts. Two additional animals received 184 and 285 X 10(6) marrow cells/kg depleted of CD34+ cells. One animal died at day 29 without engraftment, while the other had pancytopenia for greater than 100 d posttransplant. The data suggest that stem cells responsible for hematopoietic reconstitution are CD34+.

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