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Xenotropic retrovirus Bxv1 in human pancreatic β cell lines
Jeannette S. Kirkegaard, … , Claude Rescan, Olivier Albagli
Jeannette S. Kirkegaard, … , Claude Rescan, Olivier Albagli
Published February 22, 2016
Citation Information: J Clin Invest. 2016;126(3):1109-1113. https://doi.org/10.1172/JCI83573.
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Brief Report Cell biology

Xenotropic retrovirus Bxv1 in human pancreatic β cell lines

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Abstract

It has been reported that endogenous retroviruses can contaminate human cell lines that have been passaged as xenotransplants in immunocompromised mice. We previously developed and described 2 human pancreatic β cell lines (EndoC-βH1 and EndoC-βH2) that were generated in this way. Here, we have shown that B10 xenotropic virus 1 (Bxv1), a xenotropic endogenous murine leukemia virus (MuLV), is present in these 2 recently described cell lines. We determined that Bxv1 was also present in SCID mice that were used for in vivo propagation of EndoC-βH1/2 cells, suggesting that contamination occurred during xenotransplantation. EndoC-βH1/2 cells released Bxv1 particles that propagated to human 293T and Mus dunni cells. Mobilization assays demonstrated that Bxv1 transcomplements defective MuLV-based retrovectors. In contrast, common rodent β cell lines, rat INS-1E and RIN-5F cells and mouse MIN6 and βTC3 cells, displayed either no or extremely weak xenotropic helper activity toward MuLV-based retrovectors, although xenotropic retrovirus sequences and transcripts were detected in both mouse cell lines. Bxv1 propagation from EndoC-βH1/2 to 293T cells occurred only under optimized conditions and was overall poorly efficient. Thus, although our data imply that MuLV-based retrovectors should be cautiously used in EndoC-βH1/2 cells, our results indicate that an involuntary propagation of Bxv1 from these cells can be easily avoided with good laboratory practices.

Authors

Jeannette S. Kirkegaard, Philippe Ravassard, Signe Ingvarsen, Marc Diedisheim, Emilie Bricout-Neveu, Mads Grønborg, Thomas Frogne, Raphael Scharfmann, Ole D. Madsen, Claude Rescan, Olivier Albagli

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Figure 1

13F25 identifies expression of a xenotropic envelope viral protein in EndoC-βH1 cells.

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13F25 identifies expression of a xenotropic envelope viral protein in En...
(A) 13F25 mAb specifically binds to live EndoC-βH1 and not HepG2. Insets show DAPI staining. n = 10. Scale bars: 50 μM. (B) Coomassie gels showing the proteins purified by IP from EndoC-βH1 or conditioned medium using mAb 13F25 or the isotype control Ab. The proteins, indicated with arrowhead at approximately 65 kDa or arrow (after PNGase F treatment) at approximately 50 kDa, were excised for identification by MS (n = 5 and 2 for IP from cells and from medium, respectively). See complete unedited blots in the supplemental material.

Copyright © 2021 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

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