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Rho-A prenylation and signaling link epithelial homeostasis to intestinal inflammation
Rocío López-Posadas, … , Markus F. Neurath, Imke Atreya
Rocío López-Posadas, … , Markus F. Neurath, Imke Atreya
Published January 11, 2016
Citation Information: J Clin Invest. 2016;126(2):611-626. https://doi.org/10.1172/JCI80997.
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Research Article Gastroenterology

Rho-A prenylation and signaling link epithelial homeostasis to intestinal inflammation

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Abstract

Although defects in intestinal barrier function are a key pathogenic factor in patients with inflammatory bowel diseases (IBDs), the molecular pathways driving disease-specific alterations of intestinal epithelial cells (IECs) are largely unknown. Here, we addressed this issue by characterizing the transcriptome of IECs from IBD patients using a genome-wide approach. We observed disease-specific alterations in IECs with markedly impaired Rho-A signaling in active IBD patients. Localization of epithelial Rho-A was shifted to the cytosol in IBDs, and inflammation was associated with suppressed Rho-A activation due to reduced expression of the Rho-A prenylation enzyme geranylgeranyltransferase-I (GGTase-I). Functionally, we found that mice with conditional loss of Rhoa or the gene encoding GGTase-I, Pggt1b, in IECs exhibit spontaneous chronic intestinal inflammation with accumulation of granulocytes and CD4+ T cells. This phenotype was associated with cytoskeleton rearrangement and aberrant cell shedding, ultimately leading to loss of epithelial integrity and subsequent inflammation. These findings uncover deficient prenylation of Rho-A as a key player in the pathogenesis of IBDs. As therapeutic triggering of Rho-A signaling suppressed intestinal inflammation in mice with GGTase-I–deficient IECs, our findings suggest new avenues for treatment of epithelial injury and mucosal inflammation in IBD patients.

Authors

Rocío López-Posadas, Christoph Becker, Claudia Günther, Stefan Tenzer, Kerstin Amann, Ulrike Billmeier, Raja Atreya, Gionata Fiorino, Stefania Vetrano, Silvio Danese, Arif B. Ekici, Stefan Wirtz, Veronika Thonn, Alastair J.M. Watson, Cord Brakebusch, Martin Bergö, Markus F. Neurath, Imke Atreya

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Figure 1

Gene expression array comparing IECs isolated from uninflamed and inflamed gut areas of CD patients.

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Gene expression array comparing IECs isolated from uninflamed and inflam...
(A) Heat map showing differentially expressed genes in IECs (fold-change ≥ 1.5; P ≤ 0.05). (B) Top 20 regulated canonical pathways. Data are expressed as –log (P value). P value was obtained by independent sample t test (Ingenuity analysis). n = 9.
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