Senescence-associated SIN3B promotes inflammation and pancreatic cancer progression
Maïté Rielland, … , George Miller, Gregory David
Maïté Rielland, … , George Miller, Gregory David
Published April 1, 2014
Citation Information: J Clin Invest. 2014;124(5):2125-2135. https://doi.org/10.1172/JCI72619.
View: Text | PDF
Research Article Oncology

Senescence-associated SIN3B promotes inflammation and pancreatic cancer progression

Abstract

Pancreatic ductal adenocarcinoma (PDAC) is strikingly resistant to conventional therapeutic approaches. We previously demonstrated that the histone deacetylase–associated protein SIN3B is essential for oncogene-induced senescence in cultured cells. Here, using a mouse model of pancreatic cancer, we have demonstrated that SIN3B is required for activated KRAS-induced senescence in vivo. Surprisingly, impaired senescence as the result of genetic inactivation of Sin3B was associated with delayed PDAC progression and correlated with an impaired inflammatory response. In murine and human pancreatic cells and tissues, levels of SIN3B correlated with KRAS-induced production of IL-1α. Furthermore, evaluation of human pancreatic tissue and cancer cells revealed that Sin3B was decreased in control and PDAC samples, compared with samples from patients with pancreatic inflammation. These results indicate that senescence-associated inflammation positively correlates with PDAC progression and suggest that SIN3B has potential as a therapeutic target for inhibiting inflammation-driven tumorigenesis.

Authors

Maïté Rielland, David J. Cantor, Richard Graveline, Cristina Hajdu, Lisa Mara, Beatriz de Diego Diaz, George Miller, Gregory David

×

Figure 3

Sin3B deletion impairs oncogene KRAS-induced senescence in vivo.

Options: View larger image (or click on image) Download as PowerPoint
Sin3B deletion impairs oncogene KRAS-induced senescence in vivo. (A) ...
(A) Quantitative PCR for Dec1, p15INK4B, p53, and p21 mRNA expression in pancreata obtained from 6- to 8-week-old Sin3Bp+/– KraspG12D (black bars, n = 3) and Sin3Bp–/– KraspG12D (gray bars, n = 3) mice. Sin3Bp–/– KraspG12D expression is relative to Sin3Bp+/– KraspG12D expression. *P < 0.05; **P < 0.00001. (B) Cryogenic sections stained for SA–β-gal in Sin3Bp+/– KraspG12D and Sin3Bp–/– KraspG12D pancreata. (C) Immunochemistry for p-HP1γ on paraffin sections of 8-week-old Sin3Bp+/– KraspG12D and Sin3Bp–/– KraspG12D pancreata. (D) Immunochemistry for DEC1 on paraffin sections of 8-week-old Sin3Bp+/– KraspG12D and Sin3Bp–/– KraspG12D pancreata. Arrows indicate positively stained cells. Scale bars: 50 μm. (E) Percentage of DEC1-positive ductal cells at 6 to 8 weeks and at 24 weeks in Sin3Bp+/– KraspG12D and Sin3Bp–/– KraspG12D pancreata. ND, not detected. *P < 0.05.