(A) Infected peritoneal macrophages from GFP-expressing mice were activated in the presence or in absence of L-NIL. Eighteen hours after activation, time-lapse microscopy was performed for up to 48 hours. Scale bar: 20 μm. (B) A mixture of infected peritoneal macrophages from WT GFP⁺ and Nos2^–/– mice was activated. After 10 hours, time-lapse microscopy was performed for up to 48 hours. Dashed lines indicate the outline of the cells, as identified on bright-field images. Scale bar: 20 μm. (C) Mean volume of parasites contained in Nos2^–/– cells (red dots) or GFP⁺ WT cells (black dots) over time and in different conditions (separated cultures and mixed cultures with 1:1 and 1:7 WT/Nos2^–/– ratios). Volume is expressed as a percentage of initial volume. On average, 60 parasites were analyzed at each time point. Data are representative of 2 independent experiments. (D) Lethally irradiated CD45.1 C57BL/6 WT mice were reconstituted with a mixture of 50% (or 5%) CD45.1 WT and 50% (or 95%) CD45.2 Nos2^–/– BMCs. Mixed bone marrow chimeras were infected with DsRed-expressing L. major parasites in the ear dermis. Two weeks later, half of infected mice received daily injections of L-NIL over 3 consecutive days. Infected ear tissues were then harvested and analyzed by flow cytometry. The graph shows the frequency of infected cells among CD45.1 WT and CD45.2 Nos2^–/– mPhagocytes (mean ± SEM). *P < 0.05.